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鸡抗弯曲杆菌疫苗——不同载体和免疫途径的比较

Chicken Anti-Campylobacter Vaccine - Comparison of Various Carriers and Routes of Immunization.

作者信息

Kobierecka Patrycja A, Wyszyńska Agnieszka K, Gubernator Jerzy, Kuczkowski Maciej, Wiśniewski Oskar, Maruszewska Marta, Wojtania Anna, Derlatka Katarzyna E, Adamska Iwona, Godlewska Renata, Jagusztyn-Krynicka Elżbieta K

机构信息

Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw Warsaw, Poland.

Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wrocław Wrocław, Poland.

出版信息

Front Microbiol. 2016 May 19;7:740. doi: 10.3389/fmicb.2016.00740. eCollection 2016.

Abstract

Campylobacter spp, especially the species Campylobacter jejuni, are important human enteropathogens responsible for millions of cases of gastro-intestinal disease worldwide every year. C. jejuni is a zoonotic pathogen, and poultry meat that has been contaminated by microorganisms is recognized as a key source of human infections. Although numerous strategies have been developed and experimentally checked to generate chicken vaccines, the results have so far had limited success. In this study, we explored the potential use of non-live carriers of Campylobacter antigen to combat Campylobacter in poultry. First, we assessed the effectiveness of immunization with orally or subcutaneously delivered Gram-positive Enhancer Matrix (GEM) particles carrying two Campylobacter antigens: CjaA and CjaD. These two immunization routes using GEMs as the vector did not protect against Campylobacter colonization. Thus, we next assessed the efficacy of in ovo immunization using various delivery systems: GEM particles and liposomes. The hybrid protein rCjaAD, which is CjaA presenting CjaD epitopes on its surface, was employed as a model antigen. We found that rCjaAD administered in ovo at embryonic development day 18 by both delivery systems resulted in significant levels of protection after challenge with a heterologous C. jejuni strain. In practice, in ovo chicken vaccination is used by the poultry industry to protect birds against several viral diseases. Our work showed that this means of delivery is also efficacious with respect to commensal bacteria such as Campylobacter. In this study, we evaluated the protection after one dose of vaccine given in ovo. We speculate that the level of protection may be increased by a post-hatch booster of orally delivered antigens.

摘要

弯曲杆菌属细菌,尤其是空肠弯曲杆菌,是重要的人类肠道病原体,每年在全球导致数百万例胃肠道疾病。空肠弯曲杆菌是一种人畜共患病原体,被微生物污染的禽肉被认为是人类感染的主要来源。尽管已经开发并通过实验验证了多种制备鸡疫苗的策略,但迄今为止结果成效有限。在本研究中,我们探索了使用非活性弯曲杆菌抗原载体来对抗家禽体内弯曲杆菌的可能性。首先,我们评估了口服或皮下注射携带两种弯曲杆菌抗原(CjaA和CjaD)的革兰氏阳性增强基质(GEM)颗粒进行免疫的效果。这两种以GEMs为载体的免疫途径并不能防止弯曲杆菌定植。因此,接下来我们评估了使用各种递送系统进行卵内免疫的效果:GEM颗粒和脂质体。表面呈现CjaD表位的杂交蛋白rCjaAD被用作模型抗原。我们发现,在胚胎发育第18天通过这两种递送系统进行卵内注射rCjaAD,在用异源空肠弯曲杆菌菌株攻击后可产生显著水平的保护作用。实际上,家禽行业使用卵内鸡疫苗接种来保护禽类免受几种病毒性疾病的侵害。我们的研究表明,这种递送方式对于诸如弯曲杆菌这样的共生细菌也有效。在本研究中,我们评估了卵内接种一剂疫苗后的保护作用。我们推测,通过孵化后口服抗原加强免疫可能会提高保护水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1919/4872485/eeabe51464cb/fmicb-07-00740-g001.jpg

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