Godlewska Renata, Kuczkowski Maciej, Wyszyńska Agnieszka, Klim Joanna, Derlatka Katarzyna, Woźniak-Biel Anna, Jagusztyn-Krynicka Elżbieta K
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
Department of Epizootiology and Clinic for Birds and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366, Wrocław, Poland.
Appl Microbiol Biotechnol. 2016 Oct;100(20):8855-64. doi: 10.1007/s00253-016-7699-x. Epub 2016 Jul 6.
Campylobacter jejuni is the most prevalent cause of a food-borne gastroenteritis in the developed world, with poultry being the main source of infection. Campylobacter jejuni, like other Gram-negative bacteria, constitutively releases outer membrane vesicles (OMVs). OMVs are highly immunogenic, can be taken up by mammalian cells, and are easily modifiable by recombinant engineering. We have tested their usefulness for an oral (in ovo) vaccination of chickens. Four groups of 18-day-old chicken embryos (164 animals) underwent injection of wt C. jejuni OMVs or modified OMVs or PBS into the amniotic fluid. The OMVs modifications relied on overexpression of either a complete wt cjaA gene or the C20A mutant that relocates to the periplasm. Fourteen days post-hatch chicks were orally challenged with live C. jejuni strain. Cecum colonization parameters were analyzed by two-way ANOVA with Tukey post-hoc test. The wtOMVs and OMVs with wtCjaA overexpression were found to confer significant protection of chicken against C. jejuni (p = 0.03 and p = 0.013, respectively) in comparison to PBS controls and are promising candidates for further in ovo vaccine development.
空肠弯曲菌是发达国家食源性肠胃炎最常见的病因，家禽是主要感染源。与其他革兰氏阴性菌一样，空肠弯曲菌会持续释放外膜囊泡（OMV）。OMV具有高度免疫原性，可被哺乳动物细胞摄取，且易于通过重组工程进行改造。我们测试了它们用于鸡胚口服（卵内）疫苗接种的有效性。四组18日龄鸡胚（共164只动物）在羊膜腔内注射野生型空肠弯曲菌OMV、改造后的OMV或磷酸盐缓冲盐水（PBS）。OMV的改造依赖于完整野生型cjaA基因或转移至周质的C20A突变体的过表达。孵化后14天的雏鸡用活的空肠弯曲菌菌株进行口服攻毒。通过双向方差分析和Tukey事后检验分析盲肠定植参数。与PBS对照组相比，野生型OMV和过表达野生型CjaA的OMV可显著保护鸡免受空肠弯曲菌感染（p值分别为0.03和0.013），有望成为进一步进行卵内疫苗开发的候选物。
