Graduate Aerospace Laboratories, California Institute of TechnologyPasadena, CA, USA; Department of Biomedical Engineering, McGill UniversityMontreal, QC, Canada.
Graduate Aerospace Laboratories, California Institute of TechnologyPasadena, CA, USA; Institute for Astronomy, ETH ZürichZürich, Switzerland.
Front Chem. 2016 Apr 19;4:17. doi: 10.3389/fchem.2016.00017. eCollection 2016.
Digital holographic microscopy (DHM) is an emerging imaging technique that permits instantaneous capture of a relatively large sample volume. However, large volumes usually come at the expense of lower spatial resolution, and the technique has rarely been used with prokaryotic cells due to their small size and low contrast. In this paper we demonstrate the use of a Mach-Zehnder dual-beam instrument for imaging of labeled and unlabeled bacteria and microalgae. Spatial resolution of 0.3 μm is achieved, providing a sampling of several pixels across a typical prokaryotic cell. Both cellular motility and morphology are readily recorded. The use of dyes provides both amplitude and phase contrast improvement and is of use to identify cells in dense samples.
数字全息显微镜 (DHM) 是一种新兴的成像技术，可实现相对大样本体积的瞬时捕获。然而，大体积通常会牺牲空间分辨率，并且由于其体积小和对比度低，该技术很少用于原核细胞。在本文中，我们展示了马赫-曾德尔双光束仪器在标记和未标记细菌和微藻成像中的应用。实现了 0.3μm 的空间分辨率，对典型的原核细胞进行了几个像素的采样。细胞的运动和形态都可以很容易地被记录下来。染料的使用提供了幅度和相位对比度的改善，有助于识别密集样本中的细胞。
