Meza-Velázquez R, López-Márquez F, Espinosa-Padilla S, Rivera-Guillen M, Gutíerrez-Díaz N, Pérez-Armendáriz L, Rosales-González M
Facultad de Medicina, Universidad Juárez del Estado de Durango, Gómez Palacio, Durango, Mexico; Facultad de Medicina, Universidad Autonóma de Coahuila, Mexico.
Facultad de Medicina, Universidad Autonóma de Coahuila, Mexico.
Allergol Immunopathol (Madr). 2016 Sep-Oct;44(5):433-8. doi: 10.1016/j.aller.2016.01.002. Epub 2016 May 30.
It has been suggested that polymorphisms of histamine metabolising enzymes can be a risk factor for developing histamine-involving diseases. The aim of the present study is to research the possible association between two functional single nucleotide polymorphisms (SNPs): C314T in the Histamine-N-Methyl Transferase gene and C2029G in the Diamine Oxidase gene, with the severity of allergic rhinitis and the number of allergic diseases, in a group of allergic Mexican children.
We studied 154 unrelated allergic children. SNPs were analysed by RT-PCR. The total serum IgE was measured by chemiluminescence and the serum histamine by ELISA. We used logistic regression analysis to determine OR.
Patients carrying the mutant allele for any SNP had more risk to develop higher rhinitis severity or a bigger number of allergic diseases. Haplotype analysis revealed that this effect is synergistic. In patients carrying one or two mutant alleles, serum histamine levels were higher than those of patients carrying only wild alleles. Serum IgE levels were not associated with the presence of mutant alleles.
The presence of these SNPs in patients with allergic rhinitis can lead to higher serum histamine, therefore to a higher risk of developing more severe symptoms or more associated allergic diseases, even if the serum IgE remains low.
有人提出，组胺代谢酶的多态性可能是引发涉及组胺疾病的一个风险因素。本研究的目的是在一组患过敏症的墨西哥儿童中，研究组胺 - N - 甲基转移酶基因中的C314T和二胺氧化酶基因中的C2029G这两种功能性单核苷酸多态性（SNP）与过敏性鼻炎的严重程度及过敏性疾病数量之间的可能关联。
我们研究了154名无亲属关系的患过敏症儿童。通过逆转录聚合酶链反应（RT - PCR）分析SNP。采用化学发光法测定血清总IgE，用酶联免疫吸附测定法（ELISA）测定血清组胺。我们使用逻辑回归分析来确定比值比（OR）。
携带任何一种SNP突变等位基因的患者患更严重鼻炎或更多过敏性疾病的风险更高。单倍型分析表明这种效应具有协同性。携带一个或两个突变等位基因的患者血清组胺水平高于仅携带野生型等位基因的患者。血清IgE水平与突变等位基因的存在无关。
过敏性鼻炎患者中这些SNP的存在可导致血清组胺水平升高，因此即使血清IgE水平保持较低，出现更严重症状或更多相关过敏性疾病的风险也更高。
