College of Tobacco Science/National Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural University, Zhengzhou 450002, China.
Molecules. 2017 Oct 31;22(11):1866. doi: 10.3390/molecules22111866.
In this study, crude Cortex Periplocae polysaccharides (CCPPs) were extracted with water. CCPPs were decolored with AB-8 resin and deproteinated using papain-Sevage methods. Then, they were further purified and separated through DEAE-52 anion exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Three main fractions-CPP1, CPP2, and CPP3, (CPPs)-were obtained. The average molecular weights, monosaccharide analysis, surface morphology, and chemical compositions of the CPPs were investigated by high-performance gel permeation chromatography (HPGPC), gas chromatography-mass spectrometry (GC/MS), UV-vis spectroscopy, Fourier transform infrared (FT-IR) spectrum, and nuclear magnetic resonance (NMR). In addition, the antioxidant activities of these three polysaccharides were investigated. The results indicated that all of the CPPs were composed of rhamnose, arabinose, mannose, glucose, and galactose. These three polysaccharides exhibited antioxidant activities in four assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical, reducing power, and total antioxidant activity in vitro. The data indicated that these three polysaccharides could be utilized as potential natural sources of alternative additives in the functional food, cosmetics, and pharmaceutical industries.
在这项研究中，采用水从杠柳根皮中提取粗提杠柳根皮多糖（CCPPs）。用 AB-8 树脂对其进行脱色，用木瓜蛋白酶-Sevage 法进行脱蛋白。然后，通过 DEAE-52 阴离子交换层析和 Sephadex G-100 凝胶过滤层析分别对其进行进一步的纯化和分离。得到三个主要级分-CPP1、CPP2 和 CPP3。采用高效凝胶渗透色谱（HPGPC）、气相色谱-质谱（GC/MS）、紫外-可见分光光度法、傅里叶变换红外（FT-IR）光谱和核磁共振（NMR）研究了 CPPs 的平均分子量、单糖分析、表面形态和化学组成。此外，还研究了这三种多糖的抗氧化活性。结果表明，所有 CPPs 均由鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖组成。这三种多糖在四种测定方法中均表现出抗氧化活性，包括 1,1-二苯基-2-苦基肼基（DPPH）自由基、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基、还原能力和总抗氧化能力。这些数据表明，这三种多糖可以作为功能性食品、化妆品和制药工业中替代添加剂的潜在天然来源。
