Nutrition and Health Research, Allergy Group, Nestle Research Center, Lausanne, Switzerland.
Data Science Group, Nestle Research Center, Lausanne, Switzerland.
Allergy. 2016 Dec;71(12):1753-1761. doi: 10.1111/all.12951. Epub 2016 Aug 9.
Eosinophilic esophagitis (EoE) is a severe inflammatory disease of the esophagus which is characterized histologically by an eosinophilic infiltration into the esophageal tissue. The efficacy of probiotics in the context of atopic diseases has been well investigated but, to date, there has been no study which has evaluated probiotic effects on EoE inflammation. This study sought to identify a probiotic which improves esophageal inflammation in experimental EoE.
Two candidate probiotics, Lactococcus lactis NCC 2287 and Bifidobacterium lactis NCC 2818, were tested in a murine model of EoE elicited by epicutaneous sensitization with Aspergillus fumigatus protein extract. Administration of bacterial strains in drinking water was used, respectively, as a preventive or treatment measure, or continuously throughout the study. Inflammatory parameters were assessed in the esophagus, skin, and lungs after allergen challenge.
In this EoE model, supplementation with L. lactis NCC 2287 significantly decreased esophageal and bronchoalveolar eosinophilia but only when given as a therapeutic treatment. No significant effect on eosinophilia was observed when NCC 2287 was given as a preventive or a continuous intervention. NCC 2287 supplementation had no significant effect on immunoglobulin levels, skin symptom scores, or on transepidermal water loss. Supplementation with another probiotic, B. lactis NCC 2818, had no significant effect on esophageal eosinophilia.
We identified a L. lactis strain, able to attenuate esophageal eosinophilic inflammation in a preclinical model of EoE. This effect is strain specific and depends on the timing and duration of bacterial supplementation. Confirmation of these observations in human clinical trials is warranted.
嗜酸性食管炎（EoE）是一种严重的食管炎症性疾病，其组织学特征是食管组织中有嗜酸性粒细胞浸润。益生菌在特应性疾病中的疗效已经得到了很好的研究，但迄今为止，还没有研究评估益生菌对 EoE 炎症的影响。本研究旨在寻找一种能改善实验性 EoE 食管炎症的益生菌。
在由 Aspergillus fumigatus 蛋白提取物经皮致敏引起的 EoE 小鼠模型中，测试了两种候选益生菌，乳球菌 lactis NCC 2287 和双歧杆菌 lactis NCC 2818。通过饮用水给药分别作为预防或治疗措施，或在整个研究过程中连续给药。在过敏原攻击后评估食管、皮肤和肺部的炎症参数。
在这种 EoE 模型中，补充乳球菌 lactis NCC 2287 可显著降低食管和支气管肺泡嗜酸性粒细胞增多，但仅在作为治疗性治疗时才有效。当 NCC 2287 作为预防或连续干预时，对嗜酸性粒细胞增多没有显著影响。NCC 2287 补充对免疫球蛋白水平、皮肤症状评分或经皮水分丢失没有显著影响。另一种益生菌双歧杆菌 lactis NCC 2818 的补充对食管嗜酸性粒细胞增多没有显著影响。
我们鉴定出一种乳球菌菌株，能够在 EoE 的临床前模型中减轻食管嗜酸性粒细胞炎症。这种作用是菌株特异性的，取决于细菌补充的时间和持续时间。需要在人类临床试验中证实这些观察结果。
