Pouyatos B, Nemoz C, Chabrol T, Potez M, Bräuer E, Renaud L, Pernet-Gallay K, Estève F, David O, Kahane P, Laissue J A, Depaulis A, Serduc R
Inserm, U1216, F-38000 Grenoble, France.
Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, F-38000 Grenoble, France.
Sci Rep. 2016 Jun 6;6:27250. doi: 10.1038/srep27250.
Synchrotron-generated X-ray (SRX) microbeams deposit high radiation doses to submillimetric targets whilst minimizing irradiation of neighboring healthy tissue. We developed a new radiosurgical method which demonstrably transects cortical brain tissue without affecting adjacent regions. We made such image-guided SRX microtransections in the left somatosensory cortex in a rat model of generalized epilepsy using high radiation doses (820 Gy) in thin (200 μm) parallel slices of tissue. This procedure, targeting the brain volume from which seizures arose, altered the abnormal neuronal activities for at least 9 weeks, as evidenced by a decrease of seizure power and coherence between tissue slices in comparison to the contralateral cortex. The brain tissue located between transections stayed histologically normal, while the irradiated micro-slices remained devoid of myelin and neurons two months after irradiation. This pre-clinical proof of concept highlights the translational potential of non-invasive SRX transections for treating epilepsies that are not eligible for resective surgery.
同步加速器产生的X射线（SRX）微束可将高辐射剂量沉积到亚毫米级目标上，同时将对邻近健康组织的照射降至最低。我们开发了一种新的放射外科方法，该方法能明显横断皮质脑组织而不影响相邻区域。我们在全身性癫痫大鼠模型的左侧体感皮层中，使用高辐射剂量（820 Gy）对薄（200μm）的平行组织切片进行了这种图像引导的SRX微横切术。该程序针对癫痫发作起源的脑区，改变了异常神经元活动至少9周，与对侧皮层相比，癫痫发作强度和组织切片之间的相关性降低证明了这一点。横切之间的脑组织在组织学上保持正常，而照射后的微切片在照射两个月后仍无髓鞘和神经元。这种临床前概念验证突出了非侵入性SRX横切术在治疗不适合切除手术的癫痫方面的转化潜力。
