鸡蛋卵磷脂囊泡中的几何堆积限制
Geometric packing constraints in egg phosphatidylcholine vesicles.
作者信息
Huang C, Mason J T
出版信息
Proc Natl Acad Sci U S A. 1978 Jan;75(1):308-10. doi: 10.1073/pnas.75.1.308.
Abstract
This investigation presents a quantitative analysis of the asymmetric transmembrane lipid packing geometry in egg phosphatidylcholine vesicles. The analysis uses hydrodynamic and nuclear magnetic resonance data obtained for homogeneous egg phosphatidylcholine vesicles and demonstrates that the average area per lipid head group and effective length of the lipid are greater on the outer monolayer than on the inner monolayer of the egg phosphatidylcholine vesicle. Results also indicate that the bilayer of the egg phosphatidylcholine vesicles has an asymmetrical interface through its center.
摘要
本研究对卵磷脂酰胆碱囊泡中不对称跨膜脂质堆积几何结构进行了定量分析。该分析使用了从均匀的卵磷脂酰胆碱囊泡获得的流体动力学和核磁共振数据，并表明卵磷脂酰胆碱囊泡外层单分子层上每个脂质头部基团的平均面积和脂质的有效长度大于内层单分子层。结果还表明，卵磷脂酰胆碱囊泡的双层膜在其中心具有不对称界面。
相似文献
1
Geometric packing constraints in egg phosphatidylcholine vesicles.鸡蛋卵磷脂囊泡中的几何堆积限制
Proc Natl Acad Sci U S A. 1978 Jan;75(1):308-10. doi: 10.1073/pnas.75.1.308.
2
Rapid transbilayer movement of phospholipids induced by an asymmetrical perturbation of the bilayer.
Biochim Biophys Acta. 1978 Feb 2;507(1):38-47. doi: 10.1016/0005-2736(78)90372-3.
3
Effects of paramagnetic shift reagents on the 13C nuclear magnetic resonance spectra of egg phosphatidylcholine enriched with 13C in the N-methyl carbons.顺磁位移试剂对在N-甲基碳中富含¹³C的鸡蛋磷脂酰胆碱的¹³C核磁共振谱的影响。
Biochemistry. 1976 Apr 20;15(8):1635-9. doi: 10.1021/bi00653a007.
4
Single bilayer vesicles prepared without sonication. Physico-chemical properties.未经过超声处理制备的单层囊泡。物理化学性质。
Biochim Biophys Acta. 1976 Dec 2;455(2):322-31. doi: 10.1016/0005-2736(76)90308-4.
5
Calcium-induced aggregation and fusion of mixed phosphatidylcholine-phosphatidic acid vesicles as studied by 31P NMR.通过31P核磁共振研究钙诱导的磷脂酰胆碱-磷脂酸混合囊泡的聚集和融合。
Biochim Biophys Acta. 1978 Dec 19;514(2):255-63. doi: 10.1016/0005-2736(78)90296-1.
6
Outside-inside distributions and sizes of mixed phosphatidylcholine-cholesterol vesicles.
Biochim Biophys Acta. 1976 Jul 15;436(4):729-40. doi: 10.1016/0005-2736(76)90402-8.
7
Outside-inside distribution and translocation of lysophosphatidylcholine in phosphatidylcholine vesicles as determinied by 13C-NMR using (N-13CH3)-enriched lipids.使用富含（N-¹³CH₃）的脂质通过¹³C-NMR测定溶血磷脂酰胆碱在磷脂酰胆碱囊泡中的内外分布及转运。
Biochim Biophys Acta. 1977 Mar 17;465(3):443-53. doi: 10.1016/0005-2736(77)90263-2.
8
Induction of a relatively fast transbilayer movement of phosphatidylcholine in vesicles. A 13CNMR study.诱导囊泡中磷脂酰胆碱相对快速的跨双层运动。一项碳-13核磁共振研究。
Biochim Biophys Acta. 1977 Jul 14;468(2):318-26. doi: 10.1016/0005-2736(77)90124-9.
9
Magnetic resonance study of the distribution of 2,2,6,6-tetramethylpiperidine-N-oxyl in phosphatidylcholine bilayers.
Biochim Biophys Acta. 1977 Mar 17;465(3):466-70. doi: 10.1016/0005-2736(77)90265-6.
10
Transmembrane asymmetry of vesicle lipids.
J Biol Chem. 1976 Apr 10;251(7):2110-12.
引用本文的文献
1
Resonance-enhanced multiphoton ionization time-of-flight mass spectrometry for direct analysis of liposome-encapsulated compounds.用于直接分析脂质体包裹化合物的共振增强多光子电离飞行时间质谱法。
Anal Sci. 2025 Feb;41(2):173-178. doi: 10.1007/s44211-024-00683-8. Epub 2024 Nov 7.
2
Nanoelectrospray based synthesis of large, transportable membranes with integrated membrane proteins.基于纳米电喷雾的大尺寸、可传输膜的合成及其整合膜蛋白的方法。
Sci Rep. 2024 Oct 24;14(1):25192. doi: 10.1038/s41598-024-76797-w.
3
Efficacy optimization of low frequency microbubble-mediated sonoporation as a drug delivery platform to cancer cells.低频微泡介导的声孔效应作为癌细胞药物递送平台的疗效优化
Int J Pharm X. 2022 Sep 22;4:100132. doi: 10.1016/j.ijpx.2022.100132. eCollection 2022 Dec.
4
Membrane curvature and PS localize coagulation proteins to filopodia and retraction fibers of endothelial cells.膜曲率和 PS 将凝血蛋白定位到内皮细胞的丝状伪足和回缩纤维上。
Blood Adv. 2023 Jan 10;7(1):60-72. doi: 10.1182/bloodadvances.2021006870.
5
A Novel Boron Lipid to Modify Liposomal Surfaces for Boron Neutron Capture Therapy.一种新型硼脂质用于修饰脂质体表面以进行硼中子俘获治疗。
Cells. 2021 Dec 5;10(12):3421. doi: 10.3390/cells10123421.
6
Single-vesicle imaging quantifies calcium's regulation of nanoscale vesicle clustering mediated by α-synuclein.单囊泡成像量化了钙对由α-突触核蛋白介导的纳米级囊泡聚集的调节作用。
Microsyst Nanoeng. 2020 Jun 29;6:38. doi: 10.1038/s41378-020-0147-1. eCollection 2020.
7
Curvature Energetics Determined by Alchemical Simulation on Four Topologically Distinct Lipid Phases.通过对四种拓扑结构不同的脂质相的化学模拟确定曲率能量。
J Phys Chem B. 2021 Feb 25;125(7):1815-1824. doi: 10.1021/acs.jpcb.0c09458. Epub 2021 Feb 11.
8
Modeling Thrombin Generation in Plasma under Diffusion and Flow.在扩散和流动条件下的血浆中凝血酶生成的建模。
Biophys J. 2020 Jul 7;119(1):162-181. doi: 10.1016/j.bpj.2020.04.033. Epub 2020 May 19.
9
Spectrophotometric Quantification of Peroxidase with p-Phenylene-diamine for Analyzing Peroxidase-Encapsulating Lipid Vesicles.用对苯二胺分光光度法定量过氧化物酶分析包封过氧化物酶的脂质囊泡。
Anal Chem. 2017 May 16;89(10):5484-5493. doi: 10.1021/acs.analchem.7b00423. Epub 2017 May 3.
10
Joint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles.不对称脂质囊泡的联合小角X射线和中子散射数据分析
J Appl Crystallogr. 2017 Feb 28;50(Pt 2):419-429. doi: 10.1107/S1600576717000656. eCollection 2017 Apr 1.
本文引用的文献
1
[Structure of liquid-crystalline phases of different phospholipids, monoglycerides, sphingolipids in the absence or presence of water].[不同磷脂、甘油单酯、鞘脂在无水或有水情况下液晶相的结构]
J Mol Biol. 1967 May 14;25(3):363-82. doi: 10.1016/0022-2836(67)90192-1.
2
Studies on phosphatidylcholine vesicles. Formation and physical characteristics.磷脂酰胆碱囊泡的研究。形成与物理特性。
Biochemistry. 1969 Jan;8(1):344-52. doi: 10.1021/bi00829a048.
3
Bilayer structure in membranes.膜中的双层结构。
Nat New Biol. 1971 Mar 17;230(11):72-6. doi: 10.1038/newbio230072a0.
4
Studies on phosphatidylcholine vesicles. Determination of partial specific volumes by sedimentation velocity method.磷脂酰胆碱囊泡的研究。用沉降速度法测定偏比容。
J Biol Chem. 1971 Apr 25;246(8):2555-60.
5
Diffusion studies on phosphatidylcholine vesicles.磷脂酰胆碱囊泡的扩散研究。
J Am Chem Soc. 1973 Jan 10;95(1):234-9. doi: 10.1021/ja00782a042.
6
Effect of sonication on the structure of lecithin bilayers.超声处理对卵磷脂双层膜结构的影响。
Biochemistry. 1972 Nov 21;11(24):4573-81. doi: 10.1021/bi00774a024.
7
Differential interaction of cholesterol with phosphatidylcholine on the inner and outer surfaces of lipid bilayer vesicles.胆固醇与磷脂酰胆碱在脂质双层囊泡内外表面的差异相互作用。
Proc Natl Acad Sci U S A. 1974 Feb;71(2):359-62. doi: 10.1073/pnas.71.2.359.
8
Lipid bilayer phase transition: density measurements and theory.脂质双分子层相变：密度测量与理论
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3443-4. doi: 10.1073/pnas.70.12.3443.
9
Structure in the polar head region of phospholipid bilayers: A 31P [1H] nuclear Overhauser effect study.磷脂双分子层极性头部区域的结构：一项31P [1H] 核Overhauser效应研究。
Biochemistry. 1976 May 18;15(10):2121-4. doi: 10.1021/bi00655a014.
10
Transmembrane asymmetry of vesicle lipids.
J Biol Chem. 1976 Apr 10;251(7):2110-12.
Zotero 插件