Department of Pharmacy, University of Pisa, via Bonanno 6, 56126 Pisa, Italy.
Interdepartmental Research Center "Nutraceuticals and Food for Health", University of Pisa, 56100 Pisa, Italy.
Nutrients. 2023 Feb 21;15(5):1073. doi: 10.3390/nu15051073.
(1) Background: Nowadays, the health-promoting properties of extra virgin olive oil (EVOO), including the antioxidant and anti-inflammatory actions, are well recognized and mainly attributed to the different polyphenols, such as oleocanthal and oleacein. In EVOO production, olive leaves represent a high value by-product, showing a wide spectrum of beneficial effects due to the presence of polyphenols, especially oleuropein. Here we report the study of olive leaf extract (OLE)-enriched EVOO extracts, obtained by adding different percentages of OLE to EVOO in order to ameliorate their nutraceutical activities. (2) Methods: The polyphenolic content of the EVOO/OLE extracts was analyzed by HPLC and the Folin-Ciocalteau assay. For further biological testing, an 8% OLE-enriched EVOO extract was chosen. Therefore, antioxidant effects were evaluated by three different methods (DPPH, ABTS, and FRAP), and the anti-inflammatory properties were assessed in terms of cyclooxygenase activity inhibition. (3) Results: The antioxidant and anti-inflammatory profiles of the new EVOO/OLE extract are significantly improved compared to those of EVOO extract; (4) Conclusions: The combination of OLE and EVOO extract can lead to an extract enriched in terms of bioactive polyphenols and endowed with better biological properties than the singular EVOO extract. Therefore, it may represent a new complement in the nutraceutical field.
（1）背景：如今，特级初榨橄榄油（EVOO）具有促进健康的特性，包括抗氧化和抗炎作用，这已得到广泛认可，主要归因于不同的多酚，如橄榄苦苷和羟基酪醇。在 EVOO 的生产过程中，橄榄叶是一种高附加值的副产品，由于多酚的存在，特别是橄榄苦苷，表现出广泛的有益作用。在这里，我们报告了研究富含橄榄叶提取物（OLE）的 EVOO 提取物，通过向 EVOO 中添加不同比例的 OLE 来改善其营养活性。（2）方法：通过 HPLC 和 Folin-Ciocalteau 法分析 EVOO/OLE 提取物的多酚含量。为了进行进一步的生物学测试，选择了 8% OLE 富集的 EVOO 提取物。因此，通过三种不同的方法（DPPH、ABTS 和 FRAP）评估了抗氧化作用，并且根据环加氧酶活性抑制评估了抗炎特性。（3）结果：与 EVOO 提取物相比，新型 EVOO/OLE 提取物的抗氧化和抗炎特性显著改善；（4）结论：OLE 和 EVOO 提取物的组合可以导致富含生物活性多酚的提取物，并具有比单一 EVOO 提取物更好的生物学特性。因此，它可能代表营养领域的一种新补充。
