伊朗东南部扎黑丹地区CISH基因多态性与肺结核风险的关联
Association of genetic polymorphisms of CISH with the risk of pulmonary tuberculosis in Zahedan, Southeast Iran.
作者信息
Naderi Mohammad, Hashemi Mohammad, Safdari Abolhassan, Bahari Gholamreza, Taheri Mohsen
机构信息
Zahedan University of Medical Sciences, Research Center for Infectious Diseases and Tropical Medicine, Zahedan, Iran.
Zahedan University of Medical Sciences, School of Medicine, Department of Clinical Biochemistry, Zahedan, Iran.
出版信息
Braz J Infect Dis. 2016 Jul-Aug;20(4):379-83. doi: 10.1016/j.bjid.2016.05.003. Epub 2016 Jun 5.
BACKGROUND
In the current study we aimed to find out the impact of cytokine-inducible Src homology 2 domain protein (CISH) gene polymorphisms on the risk of pulmonary tuberculosis (PTB) in a sample of Iranian population.
MATERIALS AND METHODS
Polymorphisms of CISH rs2239751, rs414171, and rs6768300 were determined in 200 PTB patients and 200 healthy subjects using T-ARMS-PCR or PCR-RFLP method.
RESULTS
The results showed that rs414171 A>T genotypes significantly decreased the risk of PTB (OR=0.16, 95% CI=0.10-0.27, p<0.0001, AT vs AA; OR=0.31, 95% CI=0.14-0.68, p<0.0001, TT vs AA; OR=0.19, 95% CI=0.12-0.29, p<0.0001, AT+TT vs AA; OR=0.29, 95%CI=0.20-0.42, p<0.0001, T vs A). For rs6768300, the findings indicated that this variant decreased the risk of PTB (OR=0.52, 95% CI=0.33-0.82, p=0.005, CG vs GG; OR=0.57, 95% CI=0.38-0.87, p=0.012, C vs G). No significant association was observed between CISH rs2239751 polymorphism and risk/protection of PTB.
CONCLUSION
Our findings indicated that CISH rs414171 and rs6768300 variants might be associated with protection from PTB.
背景
在当前研究中，我们旨在探究细胞因子诱导的Src同源2结构域蛋白（CISH）基因多态性对伊朗人群样本中肺结核（PTB）发病风险的影响。
材料与方法
采用T-ARMS-PCR或PCR-RFLP方法，对200例PTB患者和200例健康受试者的CISH rs2239751、rs414171和rs6768300基因多态性进行检测。
结果
结果显示，rs414171 A>T基因型显著降低PTB发病风险（OR=0.16，95%CI=0.10-0.27，p<0.0001，AT与AA相比；OR=0.31，95%CI=0.14-0.68，p<0.0001，TT与AA相比；OR=0.19，95%CI=0.12-0.29，p<0.0001，AT+TT与AA相比；OR=0.29，95%CI=0.20-0.42，p<0.0001，T与A相比）。对于rs6768300，研究结果表明该变异降低了PTB发病风险（OR=0.52，95%CI=0.33-0.82，p=0.005，CG与GG相比；OR=0.57，95%CI=0.38-0.87，p=0.012，C与G相比）。未观察到CISH rs2239751基因多态性与PTB发病风险/保护作用之间存在显著关联。
结论
我们的研究结果表明，CISH rs414171和rs6768300变异可能与预防PTB有关。
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