Grillo Marco, Konstantinides Nikolaos, Averof Michalis
Institut de Génomique Fonctionnelle de Lyon (IGFL), École Normale Supérieure de Lyon, 32 Avenue Tony Garnier, 69007 Lyon, France.
Department of Biology, New York University, 100 Washington Square East, New York, NY 10003-6688, USA.
Curr Opin Genet Dev. 2016 Oct;40:23-31. doi: 10.1016/j.gde.2016.05.006. Epub 2016 Jun 5.
How do some animals like crabs, flatworms and salamanders regenerate entire body parts after a severe injury? Which are the mechanisms and how did that regenerative ability evolve over time? The ability to regenerate complex organs is widespread in the animal kingdom, but fundamental, centuries-old questions remain unanswered. Forward genetics approaches that were so successful in probing embryonic development are lacking in most regenerative models, and candidate gene approaches can be biased and misleading. We summarize recent progress in establishing new genetic tools and approaches to study regeneration and provide a personal perspective on the feasibility and value of establishing such tools, based on our experience with a new experimental model, the crustacean Parhyale hawaiensis.
像螃蟹、扁虫和蝾螈这样的一些动物是如何在遭受严重损伤后再生整个身体部位的？其中的机制是什么，以及这种再生能力是如何随着时间演变的？再生复杂器官的能力在动物界广泛存在，但一些基本的、存在了数百年的问题仍然没有答案。在大多数再生模型中，在探究胚胎发育方面非常成功的正向遗传学方法并不存在，而候选基因方法可能存在偏差且具有误导性。我们总结了在建立研究再生的新遗传工具和方法方面的最新进展，并基于我们在新实验模型——甲壳类动物夏威夷扁虾上的经验，就建立此类工具的可行性和价值提供个人观点。
