Serano Julia M, Martin Arnaud, Liubicich Danielle M, Jarvis Erin, Bruce Heather S, La Konnor, Browne William E, Grimwood Jane, Patel Nipam H
Department of Molecular Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
Department of Integrative Biology, University of California, Berkeley, CA 94720-3140, USA; Los Medanos College, 2700 East Leland Rd., Pittsburg, CA 94565, USA.
Dev Biol. 2016 Jan 1;409(1):297-309. doi: 10.1016/j.ydbio.2015.10.029. Epub 2015 Nov 10.
Hox genes play crucial roles in establishing regional identity along the anterior-posterior axis in bilaterian animals, and have been implicated in generating morphological diversity throughout evolution. Here we report the identification, expression, and initial genomic characterization of the complete set of Hox genes from the amphipod crustacean Parhyale hawaiensis. Parhyale is an emerging model system that is amenable to experimental manipulations and evolutionary comparisons among the arthropods. Our analyses indicate that the Parhyale genome contains a single copy of each canonical Hox gene with the exception of fushi tarazu, and preliminary mapping suggests that at least some of these genes are clustered together in the genome. With few exceptions, Parhyale Hox genes exhibit both temporal and spatial colinearity, and expression boundaries correlate with morphological differences between segments and their associated appendages. This work represents the most comprehensive analysis of Hox gene expression in a crustacean to date, and provides a foundation for functional studies aimed at elucidating the role of Hox genes in arthropod development and evolution.
Hox基因在两侧对称动物沿前后轴建立区域特征方面发挥着关键作用，并且在整个进化过程中都与形态多样性的产生有关。在此，我们报告了对夏威夷片脚类甲壳动物Parhyale hawaiensis完整Hox基因集的鉴定、表达及初步基因组特征分析。Parhyale是一个新兴的模型系统，适用于节肢动物之间的实验操作和进化比较。我们的分析表明，除了ftz基因外，Parhyale基因组中每个典型Hox基因都只有一个拷贝，初步定位表明这些基因中至少有一些在基因组中是成簇的。除少数例外情况外，Parhyale Hox基因表现出时间和空间共线性，其表达边界与体节及其相关附肢之间的形态差异相关。这项工作是迄今为止对甲壳动物Hox基因表达最全面的分析，并为旨在阐明Hox基因在节肢动物发育和进化中作用的功能研究奠定了基础。
