Institut de Génomique Fonctionnelle de Lyon, CNRS, École Normale Supérieure de Lyon, and Université Claude Bernard Lyon-1, Lyon 69007, France.
Laboratoire de Biologie et Modélisation de la Cellule, École Normale Supérieure de Lyon, 69364 Lyon, France.
Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2119297119. doi: 10.1073/pnas.2119297119. Epub 2022 Jul 1.
Regenerating animals have the ability to reproduce body parts that were originally made in the embryo and subsequently lost due to injury. Understanding whether regeneration mirrors development is an open question in most regenerative species. Here, we take a transcriptomics approach to examine whether leg regeneration shows similar temporal patterns of gene expression as leg development in the embryo, in the crustacean . We find that leg development in the embryo shows stereotypic temporal patterns of gene expression. In contrast, the dynamics of gene expression during leg regeneration show a higher degree of variation related to the physiology of individual animals. A major driver of this variation is the molting cycle. We dissect the transcriptional signals of individual physiology and regeneration to obtain clearer temporal signals marking distinct phases of leg regeneration. Comparing the transcriptional dynamics of development and regeneration we find that, although the two processes use similar sets of genes, the temporal patterns in which these genes are deployed are different and cannot be systematically aligned.
再生动物具有再生胚胎中最初形成、随后因受伤而丢失的身体部位的能力。在大多数具有再生能力的物种中，理解再生是否反映了发育是一个悬而未决的问题。在这里，我们采用转录组学的方法来研究在甲壳纲动物中，腿的再生是否表现出与胚胎中腿发育相似的时间模式的基因表达。我们发现，胚胎中的腿发育表现出特定的时间模式的基因表达。相比之下，腿再生过程中基因表达的动态变化与个体动物的生理学有关，表现出更高的变异性。这种变化的一个主要驱动因素是蜕皮周期。我们剖析了个体生理学和再生的转录信号，以获得更清晰的时间信号，标记腿再生的不同阶段。比较发育和再生的转录动力学，我们发现，尽管这两个过程使用类似的基因集，但这些基因的部署时间模式不同，不能系统地对齐。
