Honma Ryusuke, Ogasawara Satoshi, Kaneko Mika K, Fujii Yuki, Oki Hiroharu, Nakamura Takuro, Takagi Michiaki, Konnai Satoru, Kato Yukinari
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Miyagi, Japan .
2 Department of Orthopaedic Surgery, Yamagata University Faculty of Medicine , Yamagata, Japan .
Monoclon Antib Immunodiagn Immunother. 2016 Aug;35(4):186-190. doi: 10.1089/mab.2016.0016. Epub 2016 Jun 7.
Podoplanin (PDPN)/Aggrus is a type I transmembrane O-glycoprotein, which is expressed in several normal tissues including podocytes of kidney and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet; however, only bovine PDPN (bovPDPN) does not possess the platelet-aggregating activity. Although many monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, and rabbit PDPN have been established, anti-bovPDPN mAbs have not been developed. In this study, we immunized mice with the recombinant proteins of bovPDPN and developed anti-bovPDPN mAbs, which are useful in immunohistochemical analysis. One of the clones, PMab-44, is useful for detecting podocytes and lymphatic endothelial cells in normal bovine tissues. PMab-44 also detected bovPDPN specifically in flow cytometry. PMab-44 is expected to be useful for investigating the function of bovPDPN.
血小板结合蛋白（PDPN）/聚集蛋白是一种I型跨膜O-糖蛋白，在包括肾足细胞和淋巴管内皮细胞在内的多种正常组织中表达。PDPN通过与血小板上的C型凝集素样受体2（CLEC-2）结合来激活血小板聚集；然而，只有牛PDPN（bovPDPN）不具备血小板聚集活性。尽管已经制备了许多针对人PDPN、小鼠PDPN、大鼠PDPN和兔PDPN的单克隆抗体（mAb），但抗bovPDPN的单克隆抗体尚未开发出来。在本研究中，我们用bovPDPN的重组蛋白免疫小鼠，制备了抗bovPDPN单克隆抗体，这些抗体可用于免疫组织化学分析。其中一个克隆PMab-44可用于检测正常牛组织中的足细胞和淋巴管内皮细胞。PMab-44在流式细胞术中也能特异性地检测到bovPDPN。预计PMab-44将有助于研究bovPDPN的功能。
