Furusawa Yoshikazu, Yamada Shinji, Itai Shunsuke, Sano Masato, Nakamura Takuro, Yanaka Miyuki, Handa Saori, Mizuno Takuya, Maeda Ken, Fukui Masato, Harada Hiroyuki, Kaneko Mika K, Kato Yukinari
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Miyagi, Japan .
2 New Industry Creation Hatchery Center, Tohoku University , Sendai, Miyagi, Japan .
Monoclon Antib Immunodiagn Immunother. 2018 Nov;37(5):233-237. doi: 10.1089/mab.2018.0030.
Podoplanin (PDPN), a type I transmembrane glycoprotein, is expressed in several body tissues, including podocytes of renal glomerulus, type I alveolar cells of lung, and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) presented on platelets. Monoclonal antibodies (mAbs) against human-, mouse-, rat-, rabbit-, dog-, bovine-, and cat-PDPN have already been established. However, anti-horse PDPN mAbs have not yet been developed. In this study, we immunized mice with synthetic horse PDPN peptides and developed anti-horse PDPN mAbs. One of the established mAbs, PMab-202 (IgG, kappa), was specifically able to detect horse PDPN in Chinese hamster ovary/horse PDPN (CHO/horPDPN) cells in flow cytometry experiments. PMab-202 was also able to detect endogenous horse PDPN expressed in and a horse kidney cell line, FHK-Tcl3.1, in flow cytometry and Western blot analyses. PMab-202 is expected to prove useful in investigating the function of horse PDPN.
血小板结合蛋白（PDPN）是一种I型跨膜糖蛋白，在包括肾小球足细胞、肺I型肺泡细胞和淋巴管内皮细胞在内的多种身体组织中表达。PDPN通过与血小板上呈现的C型凝集素样受体-2（CLEC-2）结合来激活血小板聚集。针对人、小鼠、大鼠、兔、狗、牛和猫PDPN的单克隆抗体（mAb）已经建立。然而，抗马PDPN单克隆抗体尚未开发出来。在本研究中，我们用合成的马PDPN肽免疫小鼠并开发了抗马PDPN单克隆抗体。已建立的单克隆抗体之一PMab-202（IgG，κ）在流式细胞术实验中能够特异性检测中国仓鼠卵巢/马PDPN（CHO/horPDPN）细胞中的马PDPN。在流式细胞术和蛋白质印迹分析中，PMab-202还能够检测在马肾细胞系FHK-Tcl3.1中表达的内源性马PDPN。预计PMab-202将被证明在研究马PDPN的功能方面有用。
