Yamada Shinji, Itai Shunsuke, Nakamura Takuro, Yanaka Miyuki, Saidoh Noriko, Chang Yao-Wen, Handa Saori, Harada Hiroyuki, Kagawa Yumiko, Ichii Osamu, Konnai Satoru, Kaneko Mika K, Kato Yukinari
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .
2 Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
Monoclon Antib Immunodiagn Immunother. 2017 Oct;36(5):224-230. doi: 10.1089/mab.2017.0027. Epub 2017 Jul 24.
Podoplanin (PDPN) is expressed in several normal tissues, such as lymphatic endothelial cells, podocytes of renal glomerulus, and type I alveolar cells of lung. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. Although monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, rabbit PDPN, dog PDPN, and bovine PDPN have been established, anticat PDPN (cPDPN) mAbs have not been developed. In this study, we immunized mice with Chinese hamster ovary (CHO)-K1 cell lines expressing cPDPN, and developed anti-cPDPN mAbs. One of the clones, PMab-52 (IgM, kappa), detected cPDPN specifically in flow cytometry and Western blot analysis. PMab-52 is also useful for detecting feline squamous cell carcinoma cells in immunohistochemical analysis. PMab-52 is expected to be useful for investigating the function of cPDPN in feline carcinomas.
血小板结合蛋白（PDPN）在多种正常组织中表达，如淋巴管内皮细胞、肾小球足细胞和肺的I型肺泡细胞。PDPN通过与血小板上的C型凝集素样受体2（CLEC-2）结合来激活血小板聚集。尽管已经制备了针对人PDPN、小鼠PDPN、大鼠PDPN、兔PDPN、犬PDPN和牛PDPN的单克隆抗体（mAb），但抗猫PDPN（cPDPN）的mAb尚未开发出来。在本研究中，我们用表达cPDPN的中国仓鼠卵巢（CHO）-K1细胞系免疫小鼠，并开发出了抗cPDPN的mAb。其中一个克隆PMab-52（IgM，κ）在流式细胞术和蛋白质免疫印迹分析中能特异性检测到cPDPN。在免疫组织化学分析中，PMab-52也可用于检测猫鳞状细胞癌细胞。预计PMab-52将有助于研究cPDPN在猫科动物癌症中的功能。
