Shirouchi Bungo, Nagao Koji, Umegatani Minami, Shiraishi Aya, Morita Yukiko, Kai Shunichi, Yanagita Teruyoshi, Ogawa Akihiro, Kadooka Yukio, Sato Masao
1Laboratory of Nutrition Chemistry,Department of Bioscience and Biotechnology, Faculty of Agriculture,Graduate School,Kyushu University,6-10-1 Hakozaki,Higashi-ku,Fukuoka 812-8581,Japan.
2Laboratory of Nutrition Biochemistry,Department of Applied Biochemistry and Food Science,Saga University,1 Honjo,Saga 840-8502,Japan.
Br J Nutr. 2016 Aug;116(3):451-8. doi: 10.1017/S0007114516002245. Epub 2016 Jun 8.
Probiotic Lactobacillus gasseri SBT2055 (LG2055) reduces postprandial TAG absorption and exerts anti-obesity effects in rats and humans; however, the underlying mechanisms are not fully understood. In the present study, we addressed the mechanistic insights of the anti-obesity activity of LG2055 by feeding Sprague-Dawley rats diets containing skimmed milk fermented or not by LG2055 for 4 weeks and by analysing energy expenditure, glucose tolerance, the levels of SCFA in the caecum and serum inflammatory markers. Rats fed the LG2055-containing diet demonstrated significantly higher carbohydrate oxidation in the dark cycle (active phase for rats) compared with the control group, which resulted in a significant increase in energy expenditure. LG2055 significantly reduced cumulative blood glucose levels (AUC) compared with the control diet after 3 weeks and increased the molar ratio of butyrate:total SCFA in the caecum after 4 weeks. Furthermore, the LG2055-supplemented diet significantly reduced the levels of serum amyloid P component - an indicator of the inflammatory process. In conclusion, our results demonstrate that, in addition to the inhibition of dietary TAG absorption reported previously, the intake of probiotic LG2055 enhanced energy expenditure via carbohydrate oxidation, improved glucose tolerance and attenuated inflammation, suggesting multiple additive and/or synergistic actions underlying the anti-obesity effects exerted by LG2055.
益生菌加氏乳杆菌SBT2055（LG2055）可减少餐后甘油三酯吸收，并对大鼠和人类产生抗肥胖作用；然而，其潜在机制尚未完全明确。在本研究中，我们通过给斯普拉格-道利大鼠喂食含LG2055发酵或未发酵脱脂牛奶的日粮4周，并分析能量消耗、葡萄糖耐量、盲肠中短链脂肪酸水平和血清炎症标志物，来探讨LG2055抗肥胖活性的作用机制。与对照组相比，喂食含LG2055日粮的大鼠在暗周期（大鼠的活跃期）表现出显著更高的碳水化合物氧化，这导致能量消耗显著增加。与对照日粮相比，LG2055在3周后显著降低了累积血糖水平（AUC），并在4周后增加了盲肠中丁酸盐与总短链脂肪酸的摩尔比。此外，补充LG2055的日粮显著降低了血清淀粉样P成分的水平，这是炎症过程的一个指标。总之，我们的结果表明，除了先前报道的抑制膳食甘油三酯吸收外，摄入益生菌LG2055还通过碳水化合物氧化增强了能量消耗，改善了葡萄糖耐量并减轻了炎症，这表明LG2055发挥抗肥胖作用的潜在机制是多种相加和/或协同作用。
