猪繁殖与呼吸综合征病毒（PRRSV）的非结构蛋白11（nsp11）促进PRRSV在MARC-145细胞中的感染。
Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells.
作者信息
Shi Xibao, Zhang Xiaozhuan, Chang Yongzhe, Jiang Bo, Deng Ruiguang, Wang Aiping, Zhang Gaiping
机构信息
College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
出版信息
BMC Vet Res. 2016 Jun 6;12:90. doi: 10.1186/s12917-016-0717-5.
BACKGROUND
Porcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it. Previous studies have indicated that the nonstructural protein 11 (nsp11) of PRRSV may be an important protein for the immune escape of PRRSV.
RESULTS
Here, we firstly explored the effect of over-expression of nsp11 on PRRSV infection and found that over-expression of nsp11 enhanced the PRRSV titers while the small interfering RNA (siRNAs) specifically targeting nsp11 could reduce the PRRSV titers in MARC-145 cells.
CONCLUSION
In conclusion, PRRSV nsp11 promotes PRRSV infection in MARC-145 cells and siRNAs targeting nsp11 may be a potential therapeutic strategy to control PRRSV in future.
背景
猪繁殖与呼吸综合征病毒（PRRSV）引发了全球范围内猪最重要的毁灭性疾病之一，且目前的方法对其控制效果不佳。先前的研究表明，PRRSV的非结构蛋白11（nsp11）可能是PRRSV免疫逃逸的重要蛋白。
结果
在此，我们首先探究了nsp11过表达对PRRSV感染的影响，发现nsp11过表达提高了PRRSV滴度，而特异性靶向nsp11的小干扰RNA（siRNAs）可降低MARC-145细胞中的PRRSV滴度。
结论
总之，PRRSV nsp11促进MARC-145细胞中的PRRSV感染，靶向nsp11的siRNAs可能是未来控制PRRSV的一种潜在治疗策略。
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