Modular, Nondegenerate Polyprotein Scaffolds for Atomic Force Spectroscopy.
作者信息
Scholl Zackary N, Josephs Eric A, Marszalek Piotr E
机构信息
Computational Biology and Bioinformatics Program and §Department of Mechanical Engineering and Materials Science, Edmund T. Pratt, Jr. School of Engineering, Duke University , Durham, North Carolina, United States.
出版信息
Biomacromolecules. 2016 Jul 11;17(7):2502-5. doi: 10.1021/acs.biomac.6b00548. Epub 2016 Jun 16.
Abstract
摘要
相似文献
1
Modular, Nondegenerate Polyprotein Scaffolds for Atomic Force Spectroscopy.用于原子力光谱的模块化、非简并多蛋白支架
Biomacromolecules. 2016 Jul 11;17(7):2502-5. doi: 10.1021/acs.biomac.6b00548. Epub 2016 Jun 16.
2
The mechanical fingerprint of a parallel polyprotein dimer.平行多蛋白二聚体的机械指纹图谱。
Biophys J. 2007 Feb 15;92(4):L36-8. doi: 10.1529/biophysj.106.097741. Epub 2006 Dec 8.
3
Polyprotein synthesis: a journey from the traditional pre-translational method to modern post-translational approaches for single-molecule force spectroscopy.多聚蛋白合成：从传统的翻译前方法到现代的单分子力谱学翻译后方法的旅程。
Chem Commun (Camb). 2023 Jun 6;59(46):6946-6955. doi: 10.1039/d3cc01756g.
4
Single molecular force spectroscopy of modular proteins in the nervous system.
Neuron. 2000 Sep;27(3):435-46. doi: 10.1016/s0896-6273(00)00055-6.
5
Cysteine engineering of polyproteins for single-molecule force spectroscopy.用于单分子力谱分析的多聚蛋白的半胱氨酸工程改造
Nat Protoc. 2006;1(1):80-4. doi: 10.1038/nprot.2006.12.
6
Rapid and Robust Polyprotein Production Facilitates Single-Molecule Mechanical Characterization of β-Barrel Assembly Machinery Polypeptide Transport Associated Domains.快速而稳健的多蛋白生产有助于对β-桶组装机制多肽转运相关结构域进行单分子力学特性分析。
ACS Nano. 2015 Sep 22;9(9):8811-21. doi: 10.1021/acsnano.5b01962. Epub 2015 Aug 26.
7
Single-molecule force-clamp spectroscopy: dwell time analysis and practical considerations.单分子力钳光谱法：停留时间分析及实际考虑因素。
Langmuir. 2011 Feb 15;27(4):1440-7. doi: 10.1021/la104130n. Epub 2010 Nov 30.
8
Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level.在单分子水平上验证了多蛋白的酶生物合成和固定化。
Nat Commun. 2019 Jun 24;10(1):2775. doi: 10.1038/s41467-019-10696-x.
9
Stretching single molecules into novel conformations using the atomic force microscope.使用原子力显微镜将单个分子拉伸成新的构象。
Nat Struct Biol. 2000 Sep;7(9):719-24. doi: 10.1038/78936.
10
Force spectroscopy studies on protein-ligand interactions: a single protein mechanics perspective.蛋白质-配体相互作用的力谱研究：单蛋白力学视角
FEBS Lett. 2014 Oct 1;588(19):3613-20. doi: 10.1016/j.febslet.2014.04.009. Epub 2014 Apr 18.
引用本文的文献
1
Tandem repeats of highly bioluminescent NanoLuc are refolded noncanonically by the Hsp70 machinery.高度生物发光的 NanoLuc 串联重复序列通过 Hsp70 机制进行非canonical 重折叠。
Protein Sci. 2024 Feb;33(2):e4895. doi: 10.1002/pro.4895.
2
Detecting molecular interactions in live-cell single-molecule imaging with proximity-assisted photoactivation (PAPA).利用邻近辅助光激活（PAPA）技术在活细胞单分子成像中检测分子相互作用。
Elife. 2022 Aug 17;11:e76870. doi: 10.7554/eLife.76870.
3
Measuring biological materials mechanics with atomic force microscopy - Mechanical unfolding of biopolymers.用原子力显微镜测量生物材料力学 - 生物聚合物的机械展开。
Microsc Res Tech. 2022 Aug;85(8):3025-3036. doi: 10.1002/jemt.24136. Epub 2022 May 2.
4
A-to-I RNA editing of Filamin A regulates cellular adhesion, migration and mechanical properties.细丝蛋白 A 的 A-to-I RNA 编辑调控细胞黏附、迁移和力学特性。
FEBS J. 2022 Aug;289(15):4580-4601. doi: 10.1111/febs.16391. Epub 2022 Mar 9.
5
Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc.一种小型、高发光的工程蛋白 NanoLuc 的机械稳定性。
Int J Mol Sci. 2020 Dec 23;22(1):55. doi: 10.3390/ijms22010055.
6
Competing Pathways and Multiple Folding Nuclei in a Large Multidomain Protein, Luciferase.大型多结构域蛋白荧光素酶中的竞争途径和多个折叠核心
Biophys J. 2017 May 9;112(9):1829-1840. doi: 10.1016/j.bpj.2017.03.028.
本文引用的文献
1
Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation.通过分选酶和Sfp介导的酶促连接实现重复蛋白质聚合物的序列无关克隆和翻译后修饰。
Biomacromolecules. 2016 Apr 11;17(4):1330-8. doi: 10.1021/acs.biomac.5b01726. Epub 2016 Mar 22.
2
Single-molecule force spectroscopy on polyproteins and receptor-ligand complexes: The current toolbox.多聚蛋白和受体-配体复合物的单分子力谱：当前的工具箱
J Struct Biol. 2017 Jan;197(1):3-12. doi: 10.1016/j.jsb.2016.02.011. Epub 2016 Feb 9.
3
Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins.组合密码子重排可实现可扩展的基因合成和重复蛋白的扩增。
Nat Mater. 2016 Apr;15(4):419-24. doi: 10.1038/nmat4521. Epub 2016 Jan 4.
4
Single-molecule Force Spectroscopy Predicts a Misfolded, Domain-swapped Conformation in human γD-Crystallin Protein.单分子力谱预测人γD-晶体蛋白存在错误折叠的结构域交换构象。
J Biol Chem. 2016 Feb 19;291(8):4226-35. doi: 10.1074/jbc.M115.673871. Epub 2015 Dec 24.
5
Rapid and Robust Polyprotein Production Facilitates Single-Molecule Mechanical Characterization of β-Barrel Assembly Machinery Polypeptide Transport Associated Domains.快速而稳健的多蛋白生产有助于对β-桶组装机制多肽转运相关结构域进行单分子力学特性分析。
ACS Nano. 2015 Sep 22;9(9):8811-21. doi: 10.1021/acsnano.5b01962. Epub 2015 Aug 26.
6
Direct Observation of the Reversible Two-State Unfolding and Refolding of an α/β Protein by Single-Molecule Atomic Force Microscopy.通过单分子原子力显微镜直接观察 α/β 蛋白的可逆两态展开和重折叠。
Angew Chem Int Ed Engl. 2015 Aug 17;54(34):9921-5. doi: 10.1002/anie.201502938. Epub 2015 Jul 1.
7
Polyproteins in structural biology.结构生物学中的多聚蛋白。
Curr Opin Struct Biol. 2015 Jun;32:139-46. doi: 10.1016/j.sbi.2015.04.007. Epub 2015 May 18.
8
Life in extreme environments: single molecule force spectroscopy as a tool to explore proteins from extremophilic organisms.极端环境中的生命：单分子力谱作为探索嗜极生物蛋白质的工具
Biochem Soc Trans. 2015 Apr;43(2):179-85. doi: 10.1042/BST20140274.
9
The elastic free energy of a tandem modular protein under force.受力作用下串联模块化蛋白质的弹性自由能。
Biochem Biophys Res Commun. 2015 May 1;460(2):434-8. doi: 10.1016/j.bbrc.2015.03.051. Epub 2015 Mar 18.
10
Direct observation of multimer stabilization in the mechanical unfolding pathway of a protein undergoing oligomerization.直接观察在经历寡聚化的蛋白质的机械解折叠途径中多聚体的稳定化。
ACS Nano. 2015 Feb 24;9(2):1189-97. doi: 10.1021/nn504686f. Epub 2015 Feb 10.
Zotero 插件