Liu Xianchu, Liu Ming, Mo Yanzhi, Peng Huan, Gong Jingbo, Li Zhuang, Chen Jiaxue, Xie Jingtao
Department of Applied Psychology, Hunan University of Chinese Medicine, Hunan, PR China.
Iran J Basic Med Sci. 2016 Apr;19(4):417-22.
Previous research demonstrated that diabetes is one of the leading causes of learning and memory deficits. Naringin, a bioflavonoid isolated from grapefruits and oranges, has potent protective effects on streptozotocin (STZ)-induced diabetic rats. Recently, the effects of naringin on learning and memory performances were monitored in many animal models of cognitive impairment. However, to date, no studies have investigated the ameliorative effects of naringin on diabetes-associated cognitive decline (DACD). In this study, we investigated the effects of naringin, using a STZ-injected rat model and explored its potential mechanism.
Diabetic rats were treated with naringin (100 mg/kg/d) for 7 days. The learning and memory function were assessed by Morris water maze test. The oxidative stress indicators [superoxide dismutase (SOD) and malondialdehyde (MDA)] and inflammatory cytokines (TNF-a, IL-1β, and IL-6) were measured in hippocampus using corresponding commercial kits. The mRNA and protein levels of PPARγ were evaluated by real time (RT)-PCR and Western blot analysis.
The results showed that supplementation of naringin improved learning and memory performances compared with the STZ group. Moreover, naringin supplement dramatically increased SOD levels, reduced MDA levels, and alleviated TNF-α, IL-1β, and IL-6 compared with the STZ group in the hippocampus. The pretreatment with naringin also significantly increased PPARγ expression.
Our results showed that naringin may be a promising therapeutic agent for improving cognitive decline in DACD.
先前的研究表明，糖尿病是导致学习和记忆缺陷的主要原因之一。柚皮苷是一种从葡萄柚和橙子中分离出的生物类黄酮，对链脲佐菌素（STZ）诱导的糖尿病大鼠具有强大的保护作用。最近，在许多认知障碍动物模型中监测了柚皮苷对学习和记忆表现的影响。然而，迄今为止，尚无研究调查柚皮苷对糖尿病相关认知衰退（DACD）的改善作用。在本研究中，我们使用STZ注射大鼠模型研究了柚皮苷的作用，并探讨了其潜在机制。
糖尿病大鼠用柚皮苷（100mg/kg/d）治疗7天。通过莫里斯水迷宫试验评估学习和记忆功能。使用相应的商业试剂盒测量海马中的氧化应激指标[超氧化物歧化酶（SOD）和丙二醛（MDA）]以及炎性细胞因子（TNF-α、IL-1β和IL-6）。通过实时（RT）-PCR和蛋白质印迹分析评估PPARγ的mRNA和蛋白质水平。
结果表明，与STZ组相比，补充柚皮苷可改善学习和记忆表现。此外，与STZ组相比，补充柚皮苷可显著提高海马中的SOD水平，降低MDA水平，并减轻TNF-α、IL-1β和IL-6。用柚皮苷预处理也显著增加了PPARγ的表达。
我们的结果表明，柚皮苷可能是改善DACD认知衰退的一种有前景的治疗药物。
