Sasahira Tomonori, Kurihara Miyako, Nakashima Chie, Kirita Tadaaki, Kuniyasu Hiroki
Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
Department of Oral and Maxillofacial Surgery, Nara Medical University, Kashihara, Japan.
Br J Cancer. 2016 Jun 28;115(1):52-8. doi: 10.1038/bjc.2016.167. Epub 2016 Jun 9.
Oral squamous cell carcinomas have high potential for locoregional invasion and nodal metastasis. Thus, early detection and elucidation of detailed molecular mechanisms of OSCCs are important. Roles of LEM domain containing 1 (LEMD1), a novel cancer-testis antigen, in OSCCs are unclear.
We performed immunohistochemical analysis of LEMD1 in 289 OSCC patients and examined functions of LEMD1 in these carcinomas.
Immunohistochemical analysis showed that 101 patients were positive for LEMD1. LEM domain containing 1 expression levels in OSCCs significantly correlated with tumour progression (T factor and clinical stage), nodal metastasis, and poor prognosis. LEM domain containing 1 expression was an independent predictor of disease-free survival in OSCC patients. In OSCCs, LEMD1 knockdown suppressed cancer cell invasion. Moreover, downregulation of LEMD1 expression inhibited adhesion and transmigration of OSCCs and vascular or lymphatic vascular endothelial cells.
Our findings suggest that LEMD1 is a novel tumour progressive factor and may be a useful diagnostic and therapeutic target in OSCCs.
口腔鳞状细胞癌具有较高的局部区域侵袭和淋巴结转移潜能。因此，早期检测和阐明口腔鳞状细胞癌的详细分子机制很重要。新型癌-睾丸抗原含LEM结构域1（LEMD1）在口腔鳞状细胞癌中的作用尚不清楚。
我们对289例口腔鳞状细胞癌患者进行了LEMD1的免疫组织化学分析，并检测了LEMD1在这些癌中的功能。
免疫组织化学分析显示，101例患者LEMD1呈阳性。口腔鳞状细胞癌中含LEM结构域1的表达水平与肿瘤进展（T因子和临床分期）、淋巴结转移及预后不良显著相关。含LEM结构域1的表达是口腔鳞状细胞癌患者无病生存的独立预测指标。在口腔鳞状细胞癌中，LEMD1敲低可抑制癌细胞侵袭。此外，LEMD1表达下调抑制口腔鳞状细胞癌及血管或淋巴管内皮细胞的黏附和迁移。
我们的研究结果表明，LEMD1是一种新型肿瘤进展因子，可能是口腔鳞状细胞癌中一个有用的诊断和治疗靶点。
