使用淋巴报告基因小鼠及其胚胎干细胞对发育性、手术性和病理性淋巴管生成进行有效评估。
Efficient Assessment of Developmental, Surgical and Pathological Lymphangiogenesis Using a Lymphatic Reporter Mouse and Its Embryonic Stem Cells.
作者信息
Hong Mingu, Jung Eunson, Yang Sara, Jung Wonhyuek, Seong Young Jin, Park Eunkyung, Bramos Athanasios, Kim Kyu Eui, Lee Sunju, Daghlian George, Seo Jung In, Choi Inho, Choi In-Seon, Koh Chester J, Kobielak Agnieszka, Ying Qi-Long, Johnson Maxwell, Gardner Daniel, Wong Alex K, Choi Dongwon, Hong Young-Kwon
机构信息
Division of Plastic and Reconstructive Surgery, Department of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
出版信息
PLoS One. 2016 Jun 9;11(6):e0157126. doi: 10.1371/journal.pone.0157126. eCollection 2016.
Several lymphatic reporter mouse lines have recently been developed to significantly improve imaging of lymphatic vessels. Nonetheless, the usage of direct visualization of lymphatic vessels has not been fully explored and documented. Here, we characterized a new Prox1-tdTomato transgenic lymphatic reporter mouse line, and demonstrated how this animal tool enables the researchers to efficiently assess developmental, surgical and pathological lymphangiogenesis by direct visualization of lymphatic vessels. Moreover, we have derived embryonic stem cells from this reporter line, and successfully differentiated them into lymphatic vessels in vivo. In conclusion, these experimental tools and techniques will help advance lymphatic research.
最近开发了几种淋巴报告基因小鼠品系，以显著改善淋巴管的成像。尽管如此，淋巴管直接可视化的用途尚未得到充分探索和记录。在这里，我们对一种新的Prox1-tdTomato转基因淋巴报告基因小鼠品系进行了表征，并展示了这种动物工具如何使研究人员能够通过淋巴管的直接可视化来有效评估发育、手术和病理性淋巴管生成。此外，我们从这个报告基因品系中获得了胚胎干细胞，并成功地在体内将它们分化为淋巴管。总之，这些实验工具和技术将有助于推动淋巴研究。
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