Yoon Hyuk, Kim Sang Gyun, Kim Bo Kyoung, Shin Eun, Kim Nayoung, Lee Hyuk-Joon, Kang Gyeong Hoon, Jung Hyun Chae
Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Gut Liver. 2017 Jan 15;11(1):79-86. doi: 10.5009/gnl15585.
BACKGROUND/AIMS: To evaluate the expression of cellular inhibitor of apoptosis protein 2 () during gastric carcinogenesis after (HP) infection and after HP eradication.
We divided non-cancer patients into four groups according to the status of HP infection and atrophic gastritis (AG)/intestinal metaplasia (IM). We compared mRNA expression among these four groups and patients with HP-positive early gastric cancer (EGC) by using real-time polymerase chain reaction (PCR). We evaluated the expression of messenger RNA (mRNA)/protein by using real-time PCR/immunohistochemistry and the degree of apoptosis with a terminal deoxynucleotidyl transferasemediated nick end labeling assay before and 12 months after endoscopic submucosal dissection (ESD) in HP-positive EGC patients, regardless of whether they had undergone eradication therapy.
The expression of mRNA was significantly higher in the groups with HP(+), AG/IM(+), and HP-positive EGC than in the control, HP(+), and AG/ IM(-) groups (p<0.005). In the HP eradication group, the expression of mRNA/protein significantly decreased (p=0.006) and apoptosis increased at the 12-month follow-up after ESD. In the HP noneradication group, the aforementioned changes were not found during the same follow-up period.
The expression of increased during gastric carcinogenesis after HP infection; HP eradication in the patients who had undergone ESD for EGC reversed overexpression of and suppressed cell apoptosis.
背景/目的：评估幽门螺杆菌（HP）感染后及HP根除后胃癌发生过程中细胞凋亡抑制蛋白2（）的表达情况。
我们根据HP感染状态及萎缩性胃炎（AG）/肠化生（IM）情况将非癌症患者分为四组。通过实时聚合酶链反应（PCR）比较这四组以及HP阳性早期胃癌（EGC）患者中mRNA的表达。我们使用实时PCR/免疫组织化学评估信使核糖核酸（mRNA）/蛋白的表达，并在HP阳性EGC患者接受内镜黏膜下剥离术（ESD）前及术后12个月，使用末端脱氧核苷酸转移酶介导的缺口末端标记法评估凋亡程度，无论这些患者是否接受过根除治疗。
HP（+）、AG/IM（+）组及HP阳性EGC组中mRNA的表达显著高于对照组、HP（+）组及AG/IM（-）组（p<0.005）。在HP根除组，ESD术后12个月随访时，mRNA/蛋白的表达显著降低（p=0.006），凋亡增加。在HP未根除组，同一随访期内未发现上述变化。
HP感染后胃癌发生过程中表达增加；接受EGC的ESD治疗的患者根除HP可逆转的过表达并抑制细胞凋亡。
