Department of Food Science and Technology, College of Agriculture and Environmental Sciences, University of California, Davis, CA, USA.
Department of Food Science and Technology, College of Agriculture and Environmental Sciences, University of California, Davis, CA, USA.
Prostaglandins Leukot Essent Fatty Acids. 2018 Nov;138:71-80. doi: 10.1016/j.plefa.2016.05.004. Epub 2016 May 11.
Linoleic acid (LA, 18:2n-6) is the most abundant polyunsaturated fatty acid in the North American diet and is a precursor to circulating bioactive fatty acid metabolites implicated in brain disorders. This exploratory study tested the effects of increasing dietary LA on plasma and cerebral cortex metabolites derived from LA, its elongation-desaturation products dihomo-gamma linolenic (DGLA, 20:3n-6) acid and arachidonic acid (AA, 20:4n-6), as well as omega-3 alpha-linolenic (α-LNA, 18:3n-3), eicosapentaenoic (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). Plasma and cortex were obtained from rats fed a 0.4%, 5.2% or 10.5% energy LA diet for 15 weeks and subjected to liquid chromatography tandem mass spectrometry analysis. Total oxylipin concentrations, representing the esterified and unesterified pool, and unesterified oxylipins derived from LA and AA were significantly increased and EPA metabolites decreased in plasma at 5.2% or 10.5% energy LA compared to 0.4% energy LA. Unesterified plasma DHA metabolites also decreased at 10.5% energy LA. In cortex, total and unesterified LA and AA metabolites increased and unesterified EPA metabolites decreased at 5.2% or 10.5% LA. DGLA and α-LNA metabolites did not significantly change in plasma or cortex. Dietary LA lowering represents a feasible approach for targeting plasma and brain LA, AA, EPA or DHA-derived metabolite concentrations.
亚油酸（LA，18:2n-6）是北美饮食中最丰富的多不饱和脂肪酸，是循环生物活性脂肪酸代谢物的前体，与大脑紊乱有关。这项探索性研究测试了增加饮食中亚油酸对源自亚油酸的血浆和大脑皮层代谢物的影响，以及其延伸-去饱和产物二同型-γ亚麻酸（DGLA，20:3n-6）和花生四烯酸（AA，20:4n-6），以及ω-3α-亚麻酸（α-LNA，18:3n-3）、二十碳五烯酸（EPA，20:5n-3）和二十二碳六烯酸（DHA，22:6n-3）。从喂食 0.4%、5.2%或 10.5%能量亚油酸饮食 15 周的大鼠中获得血浆和大脑皮层，并进行液相色谱串联质谱分析。总氧化脂浓度代表酯化和非酯化池，以及源自 LA 和 AA 的非酯化氧化脂，在 5.2%或 10.5%能量 LA 时，与 0.4%能量 LA 相比，在血浆中显著增加，EPA 代谢物减少。10.5%能量 LA 时，非酯化血浆 DHA 代谢物也减少。在大脑皮层中，总和非酯化 LA 和 AA 代谢物增加，5.2%或 10.5% LA 时非酯化 EPA 代谢物减少。DGLA 和α-LNA 代谢物在血浆或大脑皮层中没有显著变化。降低饮食 LA 代表了一种可行的方法，可用于靶向血浆和大脑中的 LA、AA、EPA 或 DHA 衍生代谢物浓度。
