基于疏水 CdSe/ZnS 量子点的分子印迹聚合物的发展及应用于食品中 N(ε)-羧甲基赖氨酸的光传感检测。

Development and applications of molecularly imprinted polymers based on hydrophobic CdSe/ZnS quantum dots for optosensing of N(ε)-carboxymethyllysine in foods.

机构信息

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), 11 Fucheng Road, Beijing 100048, China.

出版信息

Food Chem. 2016 Nov 15;211:34-40. doi: 10.1016/j.foodchem.2016.05.038. Epub 2016 May 6.

DOI:10.1016/j.foodchem.2016.05.038

PMID:27283604

Abstract

A novel molecularly imprinted polymer (MIP) based on hydrophobic CdSe/ZnS quantum dots (HBQDs) was synthesized via an approach that combines MIPs for the selectivity and sensitivity with semiconductor HBQDs as optosensing material (HBQDs@MIP). Here, using a one-pot water-in-oil reverse microemulsion polymerization, the HBQDs@MIP was used as a molecular recognition element to construct an N(ε)-carboxymethyllysine (CML) optosensor. The HBQDs were coated with silica to endow the nanoparticles with high dispersibility and broad compatibility with hydrophilic analytes. Under optima conditions, the relative fluorescence intensity of the HBQDs@MIP decreased linearly with increasing CML concentration in the range 5-400μgL(-1). The proposed method enables, detection procedures to be completed within 90min, with good recoveries, ranging from 85% to 106%. It can be applied to the rapid and cost-effective monitoring of CML in food samples.

摘要

一种基于疏水 CdSe/ZnS 量子点（HBQDs）的新型分子印迹聚合物（MIP）通过一种将 MIP 的选择性和灵敏度与半导体 HBQDs 作为光感材料（HBQDs@MIP）相结合的方法合成。在这里，通过一锅水包油反向微乳液聚合，将 HBQDs@MIP 用作分子识别元件来构建 N(ε)-羧甲基赖氨酸（CML）光传感器。HBQDs 用二氧化硅包覆，赋予纳米粒子高分散性和与亲水性分析物的广泛兼容性。在最佳条件下，HBQDs@MIP 的相对荧光强度随 CML 浓度在 5-400μgL(-1)范围内线性降低。该方法可在 90min 内完成检测程序，回收率良好，范围为 85%-106%。它可用于快速、经济高效地监测食品样品中的 CML。