Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Biosens Bioelectron. 2014 May 15;55:127-32. doi: 10.1016/j.bios.2013.11.064. Epub 2013 Dec 11.
A novel molecularly imprinted polymer (MIP) based on hydrophobic CdSe quantum dots (QDs) was synthesized using a one-pot room-temperature reverse microemulsion polymerization, and this polymer was applied as a molecular recognition element to construct a ractopamine (RAC) optosensor. Here, hydrophobic CdSe QDs were first introduced to the hydrophilic analyte-imprinted polymer for highly selective and sensitive detection of RAC via the change in fluorescence intensity, because of the high-quality hydrophobic QDs with high quantum yield, sharp photoluminescence spectra and chemical and fluorescent stability. Under optimal conditions, the relative fluorescence intensity of MIP based on hydrophobic QDs decreased linearly with the increasing concentration of RAC in the range of 1.21 × 10(-9) -3.03 × 10(-6)mol L(-1) with a detection limit of 7.57 × 10(-10)mol L(-1), and the precision for five replicate detections of 1.51 × 10(-8)mol L(-1) RAC was 2.09% (relative standard deviation). The proposed method was successfully applied for the determination of trace RAC in pork samples, with good recoveries ranging from 82.79% to 97.23%.
一种基于疏水 CdSe 量子点 (QDs) 的新型分子印迹聚合物 (MIP) 是通过一锅室温反向胶束聚合合成的，该聚合物被用作分子识别元件来构建莱克多巴胺 (RAC) 光传感器。在这里，首先将疏水 CdSe QDs 引入亲水性分析物印迹聚合物中，通过荧光强度的变化，对 RAC 进行高选择性和高灵敏度检测，因为具有高量子产率、尖锐的光致发光光谱以及化学和荧光稳定性的高质量疏水 QDs。在最佳条件下，基于疏水 QDs 的 MIP 的相对荧光强度随 RAC 浓度在 1.21×10(-9) -3.03×10(-6)mol L(-1)范围内呈线性降低，检测限为 7.57×10(-10)mol L(-1)，对 1.51×10(-8)mol L(-1) RAC 的五次重复检测的精密度为 2.09%（相对标准偏差）。该方法成功应用于猪肉样品中痕量 RAC 的测定，回收率在 82.79%至 97.23%之间。
