Chang Chun-Chao, Huang Chi-Cheng, Yang Shung-Haur, Chien Chih-Cheng, Lee Chia-Long, Huang Chi-Jung
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan; Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
School of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Breast Center, Cathay General Hospital, Taipei, Taiwan; School of Medicine, Taipei Medical University, Taipei, Taiwan.
Data Brief. 2016 May 11;8:82-6. doi: 10.1016/j.dib.2016.05.010. eCollection 2016 Sep.
The growth arrest-specific 2 (GAS2) was cloned and found to be upregulated in the feces of recurrent CRC patients. This overexpressed GAS2 induced different patterns of gene expressions in CRC cells. Briefly, one cell proliferation marker, Ki-67 antigen (Ki-67), was upregulated in the cells with overexpressed GAS2, "Correlation between proliferation markers: PCNA, Ki-67, MCM-2 and antiapoptotic protein Bcl-2 in colorectal cancer" [1]. Whereas, the expression of another cell proliferation marker, proliferating cell nuclear antigen (PCNA), changed insignificantly [1]. In addition, the mRNA level of one cyclin involving in both cell cycle G1/S and G2/M transitions was also not affected by GAS2 overexpression, "Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin A" [2]. The experimental design and procedures in this article can be helpful for understanding the molecular significance of GAS2 in SW480 and SW620 CRC cells.
生长停滞特异性蛋白2（GAS2）被克隆出来，并且发现它在复发性结直肠癌患者的粪便中表达上调。这种过度表达的GAS2在结直肠癌细胞中诱导了不同的基因表达模式。简而言之，一种细胞增殖标志物，Ki-67抗原（Ki-67），在GAS2过度表达的细胞中上调，“增殖标志物：PCNA、Ki-67、MCM-2与抗凋亡蛋白Bcl-2在结直肠癌中的相关性”[1]。然而，另一种细胞增殖标志物，增殖细胞核抗原（PCNA）的表达变化不明显[1]。此外，一种参与细胞周期G1/S和G2/M转换的细胞周期蛋白的mRNA水平也不受GAS2过度表达的影响，“Cdc20和Cks指导纺锤体检查点非依赖性的细胞周期蛋白A的降解”[2]。本文中的实验设计和程序有助于理解GAS2在SW480和SW620结直肠癌细胞中的分子意义。
