Lorenz Daniel A, Garner Amanda L
Program in Chemical Biology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, Michigan 48109, USA.
Chem Commun (Camb). 2016 Jul 7;52(53):8267-70. doi: 10.1039/c6cc02894b. Epub 2016 Jun 10.
Catalytic enzyme-linked click-chemistry assays (cat-ELCCA) are an emerging class of biochemical assay. Herein we report on expanding the toolkit of cat-ELCCA to include the kinetically superior inverse-electron demand Diels-Alder (IEDDA) reaction. The result is a technology with improved sensitivity and reproducibility, enabling automated high-throughput screening.
催化酶联点击化学分析（cat-ELCCA）是一类新兴的生化分析方法。在此，我们报告了对cat-ELCCA工具集的扩展，将动力学上更优的逆电子需求狄尔斯-阿尔德（IEDDA）反应纳入其中。结果得到了一种灵敏度和重现性更高的技术，可实现自动化高通量筛选。
