Motta Victor F, Aguila Marcia B, Mandarim-DE-Lacerda Carlos A
Laboratory of Morphometry, Metabolism and Cardiovascular Disease, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, RJ, Brazil -
J Sports Med Phys Fitness. 2016 May;56(5):655-63.
Controlling obesity and other comorbidities in the population is a challenge in modern society. High-intensity interval training (HIIT) combines short periods of high-intensity exercise with long recovery periods or a low-intensity exercise. The aim was to assess the impact of HIIT in the context of diet-induced obesity in the animal model.
C57BL/6 mice were fed one of the two diets: standard chow (lean group [LE]) or a high-fat diet (obese group [OB]). After twelve weeks, the animals were divided into non-trained groups (LE-NT and OB-NT) and trained groups (LE-T and OB-T), and began an exercise protocol. For biochemical analysis of inflammatory and lipid profile, we used a colorimetric enzymatic method and an automatic spectrophotometer. One-way ANOVA was used for statistical analysis of the experimental groups with Holm-Sidak post-hoc Test. Two-way ANOVA analyzed the interactions between diet and HIIT protocol.
HIIT leads to significant reductions in body mass, blood glucose, glucose tolerance and hepatic lipid profile in T-groups compared to NT-groups. HIIT was able to reduce plasma levels of inflammatory cytokines. Additionally, HIIT improves the insulin immunodensity in the islets, reduces the adiposity and the hepatic steatosis in the T-groups. HIIT improves beta-oxidation and peroxisome proliferator-activated receptor (PPAR)-alpha and reduces lipogenesis and PPAR-gamma levels in the liver. In skeletal muscle, HIIT improves PPAR-alpha and glucose transporter-4 and reduces PPAR-gamma levels.
HIIT leads to attenuate the adverse effects caused by a chronic ingestion of a high-fat diet.
在现代社会，控制人群中的肥胖及其他合并症是一项挑战。高强度间歇训练（HIIT）将短时间的高强度运动与长时间的恢复期或低强度运动相结合。本研究旨在评估HIIT对饮食诱导肥胖动物模型的影响。
将C57BL/6小鼠分为两组喂食：标准饲料（瘦素组[LE]）或高脂饲料（肥胖组[OB]）。12周后，将动物分为未训练组（LE-NT和OB-NT）和训练组（LE-T和OB-T），并开始运动方案。对于炎症和脂质谱的生化分析，我们使用比色酶法和自动分光光度计。采用单因素方差分析对实验组进行统计分析，并进行Holm-Sidak事后检验。双向方差分析饮食与HIIT方案之间的相互作用。
与未训练组相比，HIIT使训练组的体重、血糖、糖耐量和肝脏脂质谱显著降低。HIIT能够降低血浆炎症细胞因子水平。此外，HIIT可改善胰岛中的胰岛素免疫密度，降低训练组的肥胖和肝脏脂肪变性。HIIT可改善肝脏中的β-氧化和过氧化物酶体增殖物激活受体（PPAR）-α，并降低肝脏中的脂肪生成和PPAR-γ水平。在骨骼肌中，HIIT可改善PPAR-α和葡萄糖转运蛋白-4，并降低PPAR-γ水平。
HIIT可减轻长期摄入高脂饮食所引起的不良反应。
