Ma Shuang-Gang, Yuan Shao-Peng, Hou Qi, Li Yong, Chen Xia, Yu Shi-Shan
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jun;38(11):1747-50.
Eight compounds were isolated from the leaves of Turpinia arguta by various chromatograhic techniques such as D101 macroporous resin, polyamide, Sephadex LH-20,and HPLC chromatography, and their structures were elucidated as rhoifolin (1), apigenin-7-O- [2"-O-alpha-L-rhamnopyranosyl-6"-O-alpha-L-rhamnopyranosyl] -beta-D-glucopyranoside (2), acacetin-7-O- [2"-O-alpha-L-rhamnopyranosyl-6"-O-beta-D-glucopyranosyl] -beta-D-glucopyranoside (3), acacetin-7-O- [2"-O-alpha-L-rhamnopyranosyl-6"-O-alpha-L-rhamnopyranosyl] -beta-D-glucopyranoside(neobudofficide, 4), luteolin-7-O-[2"-O-beta-D-glucopyranosyl] -beta-D-glucopyranoside (5), chrysoeiml-7-O-[2"-O-beta-D-glucopyranosyl] -beta-D-glucopyranoside (6), acacetin-7-O-alpha-L-rhamnopyranosyl-(1 --> 6) -O-beta-D-glucopyranoside (buddleoside, linarin, 7), and apigenin 6, 8-di-C-beta-D-glucopyranoside (8) on the basis of spectral data analysis. Compounds 3-8 were isolated from T. arguta for the first time. Compounds 2, 3 showed weak anti-inflammatory effect on LPS-stimulated RAW264.7 cell.
通过D101大孔树脂、聚酰胺、葡聚糖凝胶LH - 20和高效液相色谱等多种色谱技术，从山香圆叶中分离出8种化合物。基于光谱数据分析，确定它们的结构分别为异鼠李素（1）、芹菜素 - 7 - O - [2″ - O - α - L - 鼠李吡喃糖基 - 6″ - O - α - L - 鼠李吡喃糖基] - β - D - 葡萄糖吡喃糖苷（2）、刺槐素 - 7 - O - [2″ - O - α - L - 鼠李吡喃糖基 - 6″ - O - β - D - 葡萄糖吡喃糖基] - β - D - 葡萄糖吡喃糖苷（3）、刺槐素 - 7 - O - [2″ - O - α - L - 鼠李吡喃糖基 - 6″ - O - α - L - 鼠李吡喃糖基] - β - D - 葡萄糖吡喃糖苷（新醉鱼草苷，4）、木犀草素 - 7 - O - [2″ - O - β - D - 葡萄糖吡喃糖基] - β - D - 葡萄糖吡喃糖苷（5）、金合欢素 - 7 - O - [2″ - O - β - D - 葡萄糖吡喃糖基] - β - D - 葡萄糖吡喃糖苷（6）、刺槐素 - 7 - O - α - L - 鼠李吡喃糖基 -（1→6）- O - β - D - 葡萄糖吡喃糖苷（醉鱼草苷、里那苷，7）和芹菜素6,8 - 二 - C - β - D - 葡萄糖苷（8）。化合物3 - 8首次从山香圆中分离得到。化合物2、3对脂多糖刺激的RAW264.7细胞显示出较弱的抗炎作用。
