Huang Dan, Li Yang, Zhang Min, Ruan Shengli, Zhang Hongyang, Wang Yuerong, Hu Ping
Shanghai Key Laboratory of New Drug Design & Modern Engineering Center for TCM, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
J Sep Sci. 2016 Aug;39(15):2995-3001. doi: 10.1002/jssc.201600269. Epub 2016 Jul 8.
Panax ginseng has been applied in traditional Chinese medicine for over 2000 years. It is still one of the most popular herbs in recent decades. The prescribed ginseng-containing medicines consist of protopanaxadiol and protopanaxatriol ginsenosides, which are the major constituents of the herb. Minor ginsenosides at low levels in the herb, such as Rg3 and Rg5 , have attracted more rising attention than the major ones. The existing approaches to prepare Rg3 and Rg5 usually rely on either steamed red ginseng as the source or chemical/enzymatic conversion of protopanaxadiol to the targets. It is still highly desirable to effectively achieve such minor components. In this paper, a method integrated extraction of protopanaxadiol and conversion of it to Rg3 and Rg5 has been proposed. Protopanaxadiol was extracted and simultaneously converted to Rg3 and Rg5 by d,l-tartaric acid. The targets were absorbed by resins on expanded bed adsorption chromatography and were then separated from other ginsenosides in different stages. Compared with conventional methods, the developed process has advantages in shortening time consumption and improving the conversion ratio of protopanaxadiol, which is promising in directly achieving Rg3 and Rg5 from P. ginseng.
人参在传统中药中已应用了两千多年。近几十年来，它仍然是最受欢迎的草药之一。含人参的处方药由原人参二醇和原人参三醇人参皂苷组成，这些是该草药的主要成分。草药中含量较低的次要人参皂苷，如Rg3和Rg5，比主要成分受到了更多的关注。现有的制备Rg3和Rg5的方法通常要么依赖于以蒸制红参为原料，要么依赖于将原人参二醇化学/酶转化为目标产物。有效获得这些次要成分仍然非常必要。本文提出了一种将原人参二醇提取与转化为Rg3和Rg5相结合的方法。通过d，l-酒石酸提取原人参二醇并同时将其转化为Rg3和Rg5。目标产物通过膨胀床吸附色谱法被树脂吸附，然后在不同阶段与其他人参皂苷分离。与传统方法相比，所开发的工艺在缩短时间消耗和提高原人参二醇转化率方面具有优势，这对于直接从人参中获得Rg3和Rg5具有前景。
