Dinesh Murugesan, Roopan Selvaraj Mohana, Selvaraj Chinnadurai Immanuel
Plant Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore - 632 014, Tamilnadu, India.
Chemistry of Heterocycles & Natural Product Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632 014, Tamilnadu, India.
J Photochem Photobiol B. 2016 Aug;161:273-8. doi: 10.1016/j.jphotobiol.2016.05.033. Epub 2016 Jun 6.
The present study deals about the vicinity of phytochemicals present in the Phyllanthus emblica (P. emblica) seed extract. The bio-active compounds present in the methanolic seed extract have been identified using Gas Chromatography Mass Spectroscopy (GC-MS)·The antioxidant activity of P. emblica seed extract was evaluated using assistance of DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. The determination of total phenol and flavonoid substance were contemplated. Further blood clot lysis activity was also done to check the percentage of clot lysis in methanolic seed extract. The result proved that seed extract has potential application. The GCMS results of P. emblica suggest that Octyl-β-d-Glucopyranoside is present in major quantity. The work has been designed towards the degradation of 2-nitrophenol and 4-nitrophenol using P. emblica methanolic seed extract. The progress of nitrophenol degradation has been observed in UV-visible spectroscopy. At 5min duration, the 4-nitrophenol has been degraded up to 82.42%. This may be due to the presence of secondary metabolites such as alkaloids, carbohydrate and phenols in the P. emblica seed extract. The seed extract showed good scavenging activity which resulted in IC50 value of 85.92μg/mL. The total phenol and flavonoid content present in the extract were 48.242 and 12.72mg/mL. Also the seed extract showed good lysis when compared to the standard streptokinase.
本研究涉及余甘子种子提取物中植物化学物质的研究范围。采用气相色谱 - 质谱联用（GC - MS）法对甲醇提取物中的生物活性化合物进行了鉴定。利用DPPH（2,2 - 二苯基 - 1 - 苦基肼）法辅助评估余甘子种子提取物的抗氧化活性。对总酚和黄酮类物质的含量进行了测定。此外，还进行了血凝块溶解活性检测，以检查甲醇种子提取物中的血凝块溶解百分比。结果证明种子提取物具有潜在应用价值。余甘子的GC - MS结果表明，辛基 - β - D - 葡萄糖苷含量较高。本研究旨在利用余甘子甲醇种子提取物降解2 - 硝基苯酚和4 - 硝基苯酚。通过紫外 - 可见光谱观察了硝基苯酚的降解过程。在5分钟时，4 - 硝基苯酚的降解率高达82.42%。这可能是由于余甘子种子提取物中存在生物碱、碳水化合物和酚类等次生代谢产物。种子提取物表现出良好的清除活性，IC50值为85.92μg/mL。提取物中的总酚和黄酮含量分别为48.242和12.72mg/mL。与标准链激酶相比，种子提取物也表现出良好的溶解效果。
