Abdel-Wahhab Mosaad A, Salman Asmaa S, Ibrahim Mohamed I M, El-Kady Ahmed A, Abdel-Aziem Sekena H, Hassan Nabila S, Waly Ahmed I
Food Toxicology and Contaminants Department, National Research Center, Dokki, Cairo, Egypt.
Genetic and Cytology Department, National Research Center, Dokki, Cairo, Egypt.
Food Chem Toxicol. 2016 Aug;94:159-71. doi: 10.1016/j.fct.2016.06.005. Epub 2016 Jun 8.
The current study aimed to evaluate the protective role of curcumin nanoparticles loaded hydrogels (Cur-NPs-Hgs) against AFB1-induced genotoxicity in rat liver. Animals were divided into 7 treatment groups and treated orally for 3 weeks as follow: the control group, the group treated with Hgs alone (0.5 ml/rat), the groups treated with low or high dose of Cur-NPs-Hgs (100 or 200 mg/kg b.w), the group treated with AFB1 (0.125 mg/kg b.w) and the groups treated with AFB1 plus the low or high dose of Cur-NPs-Hgs. Blood ant liver samples were collected for different biochemical, genetical, histological and histochemical analysis. The results revealed that the prepared Cur-NPs have nearly spherical shape with average size of 140 ± 20 nm and negative zeta potential value of 30.7 ± 2.57 mV. The in vivo results showed that treatment with AFB1 decreased the body weight accompanied biochemical, genotoxicity and histological disturbances. The combined treatment with AFB1 and Cur-Nps-Hgs at the two tested doses succeeded to induce a significant protection against AFB1. It could be concluded that Cur-NPs-Hgs is a promise candidate to protect against AFB1-induce liver damage in the high incidence area. Moreover, Hgs are excellent candidates as drug delivery system.
本研究旨在评估负载姜黄素纳米颗粒的水凝胶（Cur-NPs-Hgs）对黄曲霉毒素B1（AFB1）诱导的大鼠肝脏遗传毒性的保护作用。将动物分为7个治疗组，并按以下方式口服给药3周：对照组、单独用水凝胶治疗的组（0.5毫升/只大鼠）、用低剂量或高剂量Cur-NPs-Hgs治疗的组（100或200毫克/千克体重）、用AFB1治疗的组（0.125毫克/千克体重）以及用AFB1加低剂量或高剂量Cur-NPs-Hgs治疗的组。采集血液和肝脏样本进行不同的生化、遗传、组织学和组织化学分析。结果显示，制备的Cur-NPs呈近球形，平均粒径为140±20纳米，zeta电位负值为30.7±2.57毫伏。体内实验结果表明，用AFB1治疗会导致体重下降，并伴有生化、遗传毒性和组织学紊乱。在两个测试剂量下，AFB1与Cur-Nps-Hgs联合治疗成功地对AFB1诱导了显著的保护作用。可以得出结论，Cur-NPs-Hgs有望成为高发病率地区预防AFB1诱导的肝损伤的候选药物。此外，水凝胶作为药物递送系统是优秀的候选者。
