He Shengjie, Mao Xinliang, Zhang Ting, Guo Xiaolei, Ge Yazhong, Ma Chungwah, Zhang Xuewu
a College of Food Science and Engineering, South China University of Technology , Guangzhou , China ;
b Infinitus (China) Company Ltd , Guangzhou , China.
J Microencapsul. 2016 Jun;33(4):344-54. doi: 10.1080/02652048.2016.1194904. Epub 2016 Jun 13.
Chinese three-striped box turtle (Cuora trifasciata), as a freshwater turtle, is used as a tonic food. The purpose of this study was to isolate peptides with cancer growth inhibition activity from trypsin-digested hydrolysates of turtle proteins. The results demonstrated that two fractions T1 and T2 exhibited good inhibition on HepG-2 and MCF-7 cancer cells, with an inhibition of 70.65-89.1%, at 500 μg/mL. Subsequently, three peptides were identified from T1 and T2, including RGVKGPR (T1-1), KLGPKGPR (T1-2), and SSPGPPVH (T2-1). By database search, T2-1 was a completely new peptide; its inhibition activity on MCF-7 cancer cells was the best, up to 70.02% at 500 μg/mL. Then, T1 and T2-1 were nanoencapsulated by chitosan. After nanoencapsulation, the inhibition percentages were 50.23% for the nanoencapsulated T1 on HepG-2 and 46.82% for the encapsulated T2-1 on MCF-7. The release experiment indicated that the encapsulated peptides could be slowly released in simulated gastrointestinal juice.
中国三线闭壳龟（Cuora trifasciata）作为一种淡水龟，被用作滋补食品。本研究的目的是从龟蛋白的胰蛋白酶消化水解物中分离出具有癌症生长抑制活性的肽。结果表明，两个组分T1和T2对HepG-2和MCF-7癌细胞表现出良好的抑制作用，在500μg/mL时抑制率为70.65 - 89.1%。随后，从T1和T2中鉴定出三种肽，包括RGVKGPR（T1-1）、KLGPKGPR（T1-2）和SSPGPPVH（T2-1）。通过数据库搜索，T2-1是一种全新的肽；其对MCF-7癌细胞的抑制活性最佳，在500μg/mL时高达70.02%。然后，T1和T2-1用壳聚糖进行纳米包封。纳米包封后，纳米包封的T1对HepG-2的抑制率为50.23%，包封的T2-1对MCF-7的抑制率为46.82%。释放实验表明，包封的肽可以在模拟胃肠液中缓慢释放。
