Kobayashi F, Sagawa N, Nanbu Y, Nakamura K, Nonogaki M, Ban C, Fujii S, Mori T
Faculty of Medicine, Department of Gynecology and Obstetrics, Kyoto University, Japan.
Am J Obstet Gynecol. 1989 May;160(5 Pt 1):1232-8. doi: 10.1016/0002-9378(89)90202-0.
To investigate the sources and biologic significance of CA 125 and CA 19-9 in amniotic fluid, immunohistochemical and biochemical localization of these tumor-associated glycoproteins in the decidua and fetal membranes was studied. Immunohistochemically, CA 125 and CA 19-9 were localized in the cytoplasm of decidua cells and amnion epithelial cells but not in the chorion and placental tissue. Biochemically, the 12,000 X g supernatant fractions of decidua and amnion tissues contained relatively large amounts of CA 125 and CA 19-9, 73% to 96% of which was present in the cytosolic fractions of these tissues. The CA 125 levels in the amniotic fluid decreased, whereas those of CA 19-9 increased with gestation, which correlated well with the respective levels in amnion tissues. These findings suggest that amnion cells produce and secrete these glycoproteins into the amniotic cavity. However, it is possible that the decidua also secretes CA 125 into the amniotic cavity through the chorion and amnion such as in the case of prolactin.
为研究羊水内CA 125和CA 19-9的来源及生物学意义，对蜕膜和胎膜中这些肿瘤相关糖蛋白进行了免疫组化及生化定位研究。免疫组化显示，CA 125和CA 19-9定位于蜕膜细胞和羊膜上皮细胞的细胞质中，而不在绒毛膜和胎盘组织中。生化分析表明，蜕膜和羊膜组织12,000 X g的上清组分中含有相对大量的CA 125和CA 19-9，其中73%至96%存在于这些组织的胞质组分中。羊水中CA 125水平随孕周增加而降低，而CA 19-9水平则升高，这与羊膜组织中的相应水平密切相关。这些发现提示羊膜细胞产生并分泌这些糖蛋白至羊膜腔。然而，蜕膜也有可能像催乳素那样通过绒毛膜和羊膜将CA 125分泌至羊膜腔。
