Asante Du-Bois, Effah-Yeboah Emmanuel, Barnes Precious, Abban Heckel Amoabeng, Ameyaw Elvis Ofori, Boampong Johnson Nyarko, Ofori Eric Gyamerah, Dadzie Joseph Budu
Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
Department of Clinical Nutrition and Dietetics, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
J Diabetes Res. 2016;2016:8252741. doi: 10.1155/2016/8252741. Epub 2016 May 16.
The young leaves of Vernonia amygdalina are often utilized as vegetable and for medicinal purpose compared to the old leaves. This study was designed to evaluate and compare the antidiabetic effects between ethanolic leaf extracts of old and young V. amygdalina on streptozotocin (STZ) induced diabetic rat for four weeks. Preliminary screening of both young and old ethanolic extracts revealed the presence of the same phytochemicals except flavonoids which was only present in the old V. amygdalina. Difference in antioxidant power between the young and old leaf extracts was statistically significant (p < 0.05). Both leaf extracts produced a significant (p < 0.05) antihyperglycaemic effect. Also results from treated rats revealed increasing effect in some haematological parameters. Similarly, the higher dose (300 mg/kg) of both extracts significantly (p < 0.05) reduced serum ALT, AST, and ALP levels as compared to the diabetic control rats. Results also showed significant (p < 0.05) decrease in LDL-C and VLDL-C in the extract-treated rats with a corresponding increase in HDL-C, as compared to the diabetic control rats. Moreover histopathological analysis revealed ameliorative effect of pathological insults induced by the STZ in the pancreas, liver, and spleen, most significantly the regeneration of the beta cells of the islets of Langerhans in treated rats.
与老叶相比，扁桃斑鸠菊的幼叶常被用作蔬菜和药用。本研究旨在评估和比较扁桃斑鸠菊老叶和幼叶乙醇提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠四周的抗糖尿病作用。对幼叶和老叶乙醇提取物的初步筛选显示，除了仅存在于老扁桃斑鸠菊中的黄酮类化合物外，两者含有相同的植物化学物质。幼叶和老叶提取物之间的抗氧化能力差异具有统计学意义（p < 0.05）。两种叶提取物均产生了显著的（p < 0.05）降血糖作用。此外，处理组大鼠的结果显示一些血液学参数有所增加。同样，与糖尿病对照大鼠相比，两种提取物的高剂量（300 mg/kg）均显著（p < 0.05）降低了血清ALT、AST和ALP水平。结果还显示，与糖尿病对照大鼠相比，提取物处理组大鼠的LDL-C和VLDL-C显著（p < 0.05）降低，HDL-C相应增加。此外，组织病理学分析显示，STZ对胰腺、肝脏和脾脏造成的病理损伤得到改善，最显著的是治疗组大鼠胰岛β细胞的再生。
