Xu Haiyan, Ma Qiong, Ma Jiannan, Wu Zhigang, Wang Yali, Ma Chaomei
Collage of Life Science, Inner Mongolia University, Huhhot, Inner Mongolia, China.
BMC Complement Altern Med. 2016 Jun 13;16:179. doi: 10.1186/s12906-016-1156-3.
Gurigumu-7 is an important traditional Mongolian medicine frequently used for liver diseases. However, the pharmacological effects and the bioactive constituents are not well understood.
This research was to use CCl4-induced liver damage in mice to investigate the hepatoprotective effects of Gurigumu-7 and the methanol eluted fraction from a DIAION column of an extract of Gurigumu-7 (MF). The chemical constituents of MF were analyzed by UPLC-MS.
Pretreated orally with MF (66, 132 and 264 mg/kg) once a day for 4 days dose-dependently suppressed CCl4-induced mice liver histopathological changes and serum aminotransferase activities (alanine transaminase: 1144.0 ± 787.2 v.s. 2461.8 ± 1072.7 U/L, p < 0.05; aspartate transaminase: 1173 ± 785.3 v.s. 2506.6 ± 1140.7 U/L, p < 0.01). MF treated group demonstrated increased levels of SOD (108.19 ± 30.32 v.s. 75.75 ± 5.37 U/mg protein, p < 0.01) but decreased levels of malonyldialdehyde (7.68 ± 1.95 v.s. 44.32 ± 16.68 nmol/mg protein, p < 0.01) compared to the CCl4 control group. More than 30 chemical constituents were quantified, and MF was found to be rich in ellagic acid (297.97 mg/g), luteolin and its glucosides (35.10 mg/g), apigenin and its glucosides (>30 mg/g), ursolic acid (14.91 mg/g), bidenoside C (8.75 mg/g), and proanthocyanidins (15.64 mg/g in proanthocyanidin A2 equivalent).
The methanol eluted fraction (MF) from a DIAION column of an extract of the Mongolian medicine-Gurigumu-7 was found to be more hepatoprotective than Gurigumu-7. The results suggested that MF is a promising bioactive fraction for the development of new hepatoprotective medicine with better formulation and quality control properties.
古日古木 - 7是一种常用于治疗肝脏疾病的重要传统蒙药。然而，其药理作用和生物活性成分尚不清楚。
本研究采用四氯化碳诱导小鼠肝损伤，以研究古日古木 - 7及其从DIAION柱洗脱的甲醇提取物（MF）的保肝作用。通过超高效液相色谱 - 质谱联用仪分析MF的化学成分。
MF（66、132和264 mg/kg）连续4天每天一次口服预处理，剂量依赖性地抑制四氯化碳诱导的小鼠肝脏组织病理学变化和血清转氨酶活性（丙氨酸转氨酶：1144.0±787.2对2461.8±1072.7 U/L，p<0.05；天冬氨酸转氨酶：1173±785.3对2506.6±1140.7 U/L，p<0.01）。与四氯化碳对照组相比，MF处理组超氧化物歧化酶水平升高（108.19±30.32对75.75±5.37 U/mg蛋白，p<0.01），但丙二醛水平降低（7.68±1.95对44.32±16.68 nmol/mg蛋白，p<0.01）。定量了30多种化学成分，发现MF富含鞣花酸（297.97 mg/g）、木犀草素及其糖苷（35.10 mg/g）、芹菜素及其糖苷（>30 mg/g）、熊果酸（14.91 mg/g）、双花连苷C（8.75 mg/g）和原花青素（以原花青素A2当量计为15.64 mg/g）。
发现蒙药古日古木 - 7提取物经DIAION柱洗脱的甲醇馏分（MF）比古日古木 - 7具有更强的保肝作用。结果表明，MF是一种有前景的生物活性馏分，可用于开发具有更好制剂和质量控制特性的新型保肝药物。
