Farzinebrahimi Reza, Mat Taha Rosna, Rashid Kamaludin A, Ali Ahmed Bakrudeen, Danaee Mahmoud, Rozali Shahril Efzueni
Institute of Biological Sciences (IBS), Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Biology Division, Center for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Evid Based Complement Alternat Med. 2016;2016:6429652. doi: 10.1155/2016/6429652. Epub 2016 May 19.
Leaf, seed, and tuber explants of C. latifolia were inoculated on MS medium supplemented with various concentrations of BAP and IBA, alone or in combinations, to achieve in vitro plant regeneration. Subsequently, antioxidant and antibacterial activities were determined from in vitro and in vivo plant developed. No response was observed from seed culture on MS media with various concentrations of PGRs. The highest percentage of callus was observed on tuber explants (94%) and leaf explants (89%) when cultured on MS media supplemented with IBA in combination with BAP. A maximum of 88% shoots per tuber explant, with a mean number of shoots (8.8 ± 1.0), were obtained on MS medium supplemented with combinations of BAP and IBA (2.5 mg L(-1)). The best root induction (92%) and mean number (7.6 ± 0.5) from tuber explants were recorded on 2.5 mg L(-1) IBA alone supplemented to MS medium. The higher antioxidant content (80%) was observed from in vivo tuber. However, tuber part from the intact plant showed higher inhibition zone in antibacterial activity compared to other in vitro and in vivo tested parts.
将宽叶吊兰的叶片、种子和块茎外植体接种在添加了不同浓度的苄氨基嘌呤（BAP）和吲哚丁酸（IBA）单独或组合的MS培养基上，以实现离体植株再生。随后，测定了离体和活体植株的抗氧化和抗菌活性。在添加不同浓度植物生长调节剂（PGR）的MS培养基上进行种子培养未观察到反应。当在添加了IBA与BAP组合的MS培养基上培养时，在块茎外植体（94%）和叶片外植体（89%）上观察到最高的愈伤组织百分比。在添加了BAP和IBA（2.5 mg L⁻¹）组合的MS培养基上，每个块茎外植体最多获得88%的芽，平均芽数为（8.8 ± 1.0）。在仅添加2.5 mg L⁻¹ IBA的MS培养基上，记录到块茎外植体最佳的生根诱导率（92%）和平均根数（7.6 ± 0.5）。在活体块茎中观察到较高的抗氧化剂含量（80%）。然而，与其他离体和活体测试部分相比，完整植株的块茎部分在抗菌活性方面表现出更高的抑菌圈。
