Babaei Nahid, Abdullah Nur Ashikin Psyquay, Saleh Ghizan, Abdullah Thohirah Lee
Islamic Azad University, Bushehr Branch, 7519619555 Bushehr, Iran.
Department of Crop Science, Faculty of Agriculture and Food Science, Universiti Putra Malaysia Bintulu Campus, Jalan Nyabau, 97008 Bintulu, Sarawak, Malaysia.
ScientificWorldJournal. 2014 Feb 27;2014:275028. doi: 10.1155/2014/275028. eCollection 2014.
A procedure was developed for in vitro propagation of Curculigo latifolia through shoot tip culture. Direct regeneration and indirect scalp induction of Curculigo latifolia were obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explants producing shoot, shoot number, and shoot length was obtained on MS medium supplemented with combination of thidiazuron (0.5 mg L(-1)) and indole-3-butyric acid (0.25 mg L(-1)) after both 10 and 14 weeks of cultures. Indole-3-butyric acid in combination with thidiazuron exhibited a synergistic effect on shoot regeneration. The shoot tips were able to induce maximum scalp from basal end of explants on the medium with 2 mg L(-1) thidiazuron. Cultures showed that shoot number, shoot length, and scalp size increased significantly after 14 weeks of culture. Transferring of the shoots onto the MS medium devoid of growth regulators resulted in the highest percentage of root induction and longer roots, while medium supplemented with 0.25 mg L(-1) IBA produced more numbers of roots.
已开发出一种通过茎尖培养对宽叶仙茅进行离体繁殖的方法。从在添加了不同浓度和组合的噻苯隆和吲哚-3-丁酸的MS培养基上生长的茎尖获得了宽叶仙茅的直接再生和间接愈伤组织诱导。在培养10周和14周后，在添加了噻苯隆（0.5 mg L(-1)）和吲哚-3-丁酸（0.25 mg L(-1)）组合的MS培养基上，就产生芽的外植体百分比、芽数和芽长度而言，直接再生获得了最大响应。吲哚-3-丁酸与噻苯隆组合对芽再生表现出协同作用。在含有2 mg L(-1)噻苯隆的培养基上，茎尖能够从外植体基部诱导出最大量的愈伤组织。培养表明，培养14周后，芽数、芽长度和愈伤组织大小显著增加。将芽转移到不含生长调节剂的MS培养基上，生根诱导百分比最高且根更长，而添加0.25 mg L(-1) IBA的培养基产生更多数量的根。
