利巴韦林的高效液相色谱（HPLC）测定法及HPLC测定法与放射免疫测定法的比较。
High-performance liquid chromatography (HPLC) assay for ribavirin and comparison of the HPLC assay with radioimmunoassay.
作者信息
Granich G G, Krogstad D J, Connor J D, Desrochers K L, Sherwood C
机构信息
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri.
出版信息
Antimicrob Agents Chemother. 1989 Mar;33(3):311-5. doi: 10.1128/AAC.33.3.311.
Abstract
The use of high-performance liquid chromatography (HPLC) to measure ribavirin in serum and other biological fluids has been limited by endogenous interfering substances. We report an HPLC procedure based on the extraction of ribavirin from serum, plasma, or cerebrospinal fluid with a boronate affinity gel, which uses a 3-methylcytidine internal standard. This assay is sensitive (to 0.4 microM), specific (no interference with 34 commonly prescribed drugs), reproducible (coefficients of variation from 5.4 to 22.4%), and linear (r = 0.999) over the range of clinically relevant concentrations in serum (from 0.5 to 50.0 microM). It also correlates well with the ribavirin radioimmunoassay (r = 0.992). This HPLC assay should be useful for measuring ribavirin in serum and other body fluids during clinical trials.
摘要
高效液相色谱法（HPLC）用于测定血清及其他生物体液中的利巴韦林，受到内源性干扰物质的限制。我们报告了一种基于用硼酸亲和凝胶从血清、血浆或脑脊液中提取利巴韦林的HPLC方法，该方法使用3-甲基胞苷作为内标。该测定法灵敏（检测限为0.4 microM）、特异（不受34种常用处方药干扰）、可重现（变异系数为5.4%至22.4%），并且在血清中临床相关浓度范围（0.5至50.0 microM）内呈线性（r = 0.999）。它与利巴韦林放射免疫测定法也具有良好的相关性（r = 0.992）。这种HPLC测定法在临床试验期间用于测定血清及其他体液中的利巴韦林应很有用。
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