Tankovic J, Leclercq R, Duval J
Servíce de Bactériologie-Virologie-Hygiène, Hôpital Henri Mondor, Université Paris XII, Créteil, France.
Antimicrob Agents Chemother. 1993 Apr;37(4):789-92. doi: 10.1128/AAC.37.4.789.
We determined the MICs of 28 antimicrobial agents against 36 clinical strains of Pediococcus spp. (25 P. acidilactici, 9 P. pentosaceus, and 2 P. urinaeequi strains). Penicillin G, imipenem, gentamicin, netilmicin, erythromycin, clindamycin, rifampin, chloramphenicol, daptomycin, and ramoplanin were the most active. All strains of P. acidilactici were susceptible to novobiocin, whereas all isolates of P. pentosaceus were resistant. Novobiocin could therefore be helpful for differentiation of these two closely related species. P. acidilactici HM3020 was inducibly resistant to macrolide, lincosamide, and streptogramin B-type (MLS) antibiotics. Resistance was due to a determinant homologous to ermAM and carried by a nontransferable 46-kb plasmid, pVM20. This plasmid was structurally distinct from two enterococcal MLS resistance plasmids, pIP819 and pAM beta 1. The 34 strains of P. acidilactici and P. pentosaceus were resistant to tetracycline, and total DNA of these strains did not hybridize to probes specific for tetK, tetL, tetM, and tetO.
我们测定了28种抗菌药物对36株嗜热栖热放线菌临床菌株（25株嗜酸乳杆菌、9株戊糖片球菌和2株尿道片球菌）的最低抑菌浓度（MIC）。青霉素G、亚胺培南、庆大霉素、奈替米星、红霉素、克林霉素、利福平、氯霉素、达托霉素和雷莫拉宁活性最强。所有嗜酸乳杆菌菌株对新生霉素敏感，而所有戊糖片球菌分离株均耐药。因此，新生霉素有助于区分这两个密切相关的物种。嗜酸乳杆菌HM3020对大环内酯类、林可酰胺类和链阳菌素B型（MLS）抗生素呈诱导性耐药。耐药性是由一个与ermAM同源的决定簇引起的，该决定簇由一个不可转移的46 kb质粒pVM20携带。该质粒在结构上与两种肠球菌MLS耐药质粒pIP819和pAM beta 1不同。34株嗜酸乳杆菌和戊糖片球菌对四环素耐药，这些菌株的总DNA与tetK、tetL、tetM和tetO特异性探针不杂交。
