Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Viruses. 2019 Jan 26;11(2):104. doi: 10.3390/v11020104.
is an opportunistic pathogen that is clinically associated with various human and animal diseases. In this study, the first identified phage, vB_AviM_AVP (abbreviated as AVP), was isolated and studied. AVP belongs to the family Myoviridae. AVP harbors a double-stranded DNA genome with a length of 133,806 bp and a G + C content of 34.51%. The genome sequence of AVP showed low similarity (<1% identity) to those of other phages, bacteria, or other organisms in the database. Among 165 predicted open reading frames (ORFs), there were only 69 gene products exhibiting similarity (≤65% identity) to proteins of known functions in the database. In addition, the other 36 gene products did not match any viral or prokaryotic sequences in any publicly available database. On the basis of the putative functions of the ORFs, the genome of AVP was divided into three modules: nucleotide metabolism and replication, structural components, and lysis. A phylogenetic analysis of the terminase large subunits and capsid proteins indicated that AVP represents a novel branch of phages. The observed characteristics of AVP indicate that it represents a new class of phages.
是一种机会性病原体，与人类和动物的多种疾病有关。在本研究中，首次分离并研究了一种噬菌体 vB_AviM_AVP（简称 AVP）。AVP 属于肌尾噬菌体科。AVP 含有一个长度为 133806bp 的双链 DNA 基因组，GC 含量为 34.51%。AVP 的基因组序列与数据库中的其他噬菌体、细菌或其他生物体的序列相似度较低（<1%的同一性）。在 165 个预测的开放阅读框（ORFs）中，只有 69 个基因产物与数据库中具有已知功能的蛋白质具有相似性（≤65%的同一性）。此外，其他 36 个基因产物与任何公共数据库中的任何病毒或原核序列都不匹配。根据 ORFs 的推测功能，AVP 的基因组被分为三个模块：核苷酸代谢和复制、结构成分和裂解。末端酶大亚基和衣壳蛋白的系统发育分析表明，AVP 代表了噬菌体的一个新分支。AVP 的观察特征表明它代表了一类新的噬菌体。
