Kucukaydın Zehra, Duran Cevdet, Basaran Mustafa, Camlica Fatos, Erdem Sami Said, Basaran Ahmet, Kutlu Orkide, Burnik Ferda Sevimli, Elmas Halis, Gonen Mustafa Sait
Division of Obstetrics and Gynecology, Konya Training and Research Hospital, Konya, Turkey.
Division of Endocrinology and Internal Medicine, Division of Endocrinology and Metabolism, Konya Training and Research Hospital, Meram Yeniyol, 42100, Meram, Konya, Turkey.
J Endocrinol Invest. 2016 Oct;39(10):1139-48. doi: 10.1007/s40618-016-0498-5. Epub 2016 Jun 14.
Insulin resistance (IR) and increased oxidative stress (OS) are the characteristics of polycystic ovary syndrome (PCOS). In this study, we aimed to evaluate the effects of oral glucose tolerance (OGTT) and mixed meal tests (MMT) on plasma total oxidant (TOS) and total antioxidant status (TAS) in patients with PCOS and the relationship between these parameters and IR, calculated via homeostasis of model assessment-IR (HOMA-IR) and Matsuda's insulin sensitivity index (ISI) derived from OGTT and MMT.
Twenty-two patients with PCOS, and age- and body mass index (BMI)-matched 20 women as controls were enrolled into the study. Five-hour OGTT and MMT were performed on different days, and before and after these tests, plasma TOS and TAS levels were investigated. IR was calculated with HOMA-IR and Matsuda's ISI.
HOMA-IR levels were higher in patients with PCOS, compared to controls, while Matsuda's ISI derived from OGTT and MMT was higher in controls. Plasma TOS levels before OGTT and MMT were higher in patients with PCOS than controls, while TAS levels were similar. After OGTT, plasma TOS levels became decreased at 5th hour, when compared to baseline values in PCOS group. Likewise, the same decrement was found in controls, but the decrement was not significant. After OGTT and MMT at 5th hour, no changes were observed in TAS levels, compared to baseline.
Matsuda's ISIs derived from OGTT and MMT can be used instead of each other, and interestingly, we found a decrease in TOS levels after OGTT in patients with PCOS.
胰岛素抵抗（IR）和氧化应激增加（OS）是多囊卵巢综合征（PCOS）的特征。在本研究中，我们旨在评估口服葡萄糖耐量试验（OGTT）和混合餐试验（MMT）对PCOS患者血浆总氧化剂（TOS）和总抗氧化状态（TAS）的影响，以及这些参数与通过模型评估-IR（HOMA-IR）稳态计算的IR和从OGTT及MMT得出的松田胰岛素敏感性指数（ISI）之间的关系。
本研究纳入了22例PCOS患者以及20名年龄和体重指数（BMI）匹配的女性作为对照。在不同日期进行了5小时的OGTT和MMT，并在这些试验前后检测血浆TOS和TAS水平。通过HOMA-IR和松田ISI计算IR。
与对照组相比，PCOS患者的HOMA-IR水平更高，而从OGTT和MMT得出的松田ISI在对照组中更高。PCOS患者在OGTT和MMT前的血浆TOS水平高于对照组，而TAS水平相似。在PCOS组中，OGTT后第5小时血浆TOS水平与基线值相比有所下降。同样，对照组也有相同程度的下降，但下降不显著。在OGTT和MMT后第5小时，与基线相比，TAS水平未观察到变化。
从OGTT和MMT得出的松田ISI可相互替代使用，有趣的是，我们发现PCOS患者在OGTT后TOS水平降低。
