Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Maternal, Fetal and Neonatal Research Center, Tehran University of Medical Sciences, Tehran, Iran.
J Endocrinol Invest. 2017 Jul;40(7):779-784. doi: 10.1007/s40618-017-0632-z. Epub 2017 Mar 8.
Polycystic ovary syndrome (PCOS) is the most common cause of chronic anovulation with a prevalence of 5-10% in women of reproductive age. The etiology of this disease is not well known, and hepcidin is one of the factors affecting the pathogenesis of the disease. The aim of this study was to evaluate plasma levels of hepcidin in patients with PCOS and its correlation with serum iron level.
In this case-control study, plasma levels of hepcidin, IL-6, and ferritin using ELISA method and serum iron levels using a spectrophotometric method were tested on 56 women with PCOS (case group) and 41 healthy subjects (control group). The results were analyzed using t test, General Linear Model, Binary logistic regression, and linear regression tests.
The mean hepcidin levels were 1.97 ± 0.53 and 2.40 ± 0.25 pg/ml in the case and control groups, respectively. The t-test results showed significant difference between the two groups (p = 0.0001). The mean serum iron levels were 72.89 ± 28.97 and 70.62 ± 31.18 g/dl in the case and control groups, respectively. The t test analysis indicated no significant difference between the two groups. The serum ferritin and iron levels had no significant relation with serum hepcidin level in two groups.
Despite the differences in the serum levels of hepcidin between the two groups, no significant relation was observed between serum iron levels and hepcidin level in this group of patients. This implies the need for more comprehensive studies on gene expression in hepcidin and iron pathways using real-time and Western techniques to investigate more precisely serum hepcidin level and its relationship with the factors mentioned.
多囊卵巢综合征（PCOS）是最常见的慢性排卵障碍病因，在育龄妇女中的发病率为 5-10%。其病因尚不清楚，铁调素是影响疾病发病机制的因素之一。本研究旨在评估多囊卵巢综合征患者铁调素的血浆水平及其与血清铁水平的相关性。
在这项病例对照研究中，采用酶联免疫吸附法（ELISA）检测了 56 例多囊卵巢综合征患者（病例组）和 41 例健康受试者（对照组）的血浆铁调素、IL-6 和铁蛋白水平，采用分光光度法检测血清铁水平。采用 t 检验、一般线性模型、二项逻辑回归和线性回归检验对结果进行分析。
病例组和对照组的铁调素水平均值分别为 1.97±0.53pg/ml 和 2.40±0.25pg/ml。t 检验结果显示两组间存在显著差异（p=0.0001）。病例组和对照组的血清铁水平均值分别为 72.89±28.97g/dl 和 70.62±31.18g/dl。t 检验分析表明两组间无显著差异。两组患者的血清铁蛋白和铁水平与血清铁调素水平无显著相关性。
尽管两组患者血清铁调素水平存在差异，但本研究组患者的血清铁水平与铁调素水平之间未观察到显著关系。这表明需要使用实时和 Western 技术对铁调素和铁途径的基因表达进行更全面的研究，以更精确地研究血清铁调素水平及其与上述因素的关系。
