肠道病毒而非细小病毒 B19 与特发性扩张型心肌病和心肌内 CD3、CD68 或 HLA-DR 表达有关。
Enterovirus but not Parvovirus B19 is associated with idiopathic dilated cardiomyopathy and endomyocardial CD3, CD68, or HLA-DR expression.
机构信息
Laboratoirede Virologie médicale et moléculaire, Centre hospitalier universitaire de Reims, France.
EA-4684 Cardiovir, Faculté de médecine, Université de Reims Champagne-Ardenne, Reims, France.
出版信息
J Med Virol. 2017 Jan;89(1):55-63. doi: 10.1002/jmv.24600. Epub 2016 Jun 21.
We assessed Enterovirus (EV) &Parvovirus B19 (PVB19) genomes and CD3, CD68&HLA-DR detection in dilated cardiomyopathies (DCM). EV&PVB19 genomes and CD3, CD68&HLA-DR were detected by PCR and immunohistochemistry assays in 115 endomyocardial biopsies obtained in 13 idiopathic DCM (iDCM) and 10 explained DCM (eDCM) patients. Results were compared with those of 47 atrial surgical samples (47 surgery controls) and 22 autoptic cardiac samples (11 healthy heart controls) (2008-2014, Reims, France). EV was detected in 23.1% of iDCM patients but not in eDCM and controls (P = 0.003) (viral load 803 copies/μg). PVB19 was detected in 76.9%, 80.0%, 63.6% and 78.2% of iDCM, eDCM, healthy heart and surgery controls (P = 0.99) with a mean viral load of 413, 346, 1,428, and 71 copies/μg. CD3, CD68 or HLA-DR were detected in 100 and 50% of EV and PVB19 "mono-infected" iDCM patients. EV was exclusively detected in iDCM cases in association with CD3, CD68, or HLA-DR indicating that EV could be an etiological cause in a subset of iDCM cases. By contrast the equal frequent detection of PVB19 in iDCM cases and controls without association with CD3, CD68, or HLA-DR suggested that PVB19 could be a bystander in many DCM cases. J. Med. Virol. 89:55-63, 2017. © 2016 Wiley Periodicals, Inc.
我们评估了肠道病毒（EV）和微小病毒 B19（PVB19）基因组以及扩张型心肌病（DCM）中的 CD3、CD68 和 HLA-DR 的检测。通过聚合酶链反应和免疫组织化学检测，在 13 例特发性扩张型心肌病（iDCM）和 10 例可解释的扩张型心肌病（eDCM）患者的 115 份心内膜心肌活检中检测到 EV 和 PVB19 基因组以及 CD3、CD68 和 HLA-DR。将结果与 47 例心房手术样本（47 例手术对照）和 22 例尸检心脏样本（11 例健康心脏对照）进行比较（2008-2014 年，法国兰斯）。EV 在 23.1%的 iDCM 患者中被检测到，但在 eDCM 和对照组中未被检测到（P=0.003）（病毒载量为 803 拷贝/μg）。PVB19 在 76.9%、80.0%、63.6%和 78.2%的 iDCM、eDCM、健康心脏和手术对照组中被检测到，平均病毒载量分别为 413、346、1428 和 71 拷贝/μg。CD3、CD68 或 HLA-DR 在 100%和 50%的 EV 和 PVB19“单感染”iDCM 患者中被检测到。EV 仅在与 CD3、CD68 或 HLA-DR 相关的 iDCM 病例中被检测到，这表明 EV 可能是一组 iDCM 病例的病因。相比之下，PVB19 在 iDCM 病例和对照组中同样频繁地被检测到，而与 CD3、CD68 或 HLA-DR 无关，这表明 PVB19 在许多 DCM 病例中可能是旁观者。J. Med. Virol. 89:55-63, 2017。版权所有 2016 年 Wiley 期刊有限公司。
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