Department of Biochemistry, Faculty of Medicine, University of Niš, Bulevar dr Zorana Đinđića 81, 18000 Niš, Serbia.
Department of Pathology, Faculty of Medicine, University of Niš, Bulevar dr Zorana Đinđića 81, 18000 Niš, Serbia.
Life Sci. 2016 Jul 15;157:168-177. doi: 10.1016/j.lfs.2016.06.007. Epub 2016 Jun 14.
The aim of this research was to determine the hepatoprotective effects of anthocyanins from bilberry extract in rats exposed to carbon tetrachloride (CCl4) by monitoring the parameters of oxidative stress and apoptosis, and by performing the histopathological and morphometric analyses.
Animals were divided into four groups: Group I (0.9% NaCl-10days), Group II (bilberry extract, 75mg/kg-10days), Group III (0,9% NaCl-9days, and on the tenth day CCl4-2ml/kg), Group IV (bilberry extract, 75mg/kg-10days and on the tenth day CCl4-2ml/kg).
Bilberry extract led to a significant decrease in the activity of biochemical parameters in serum (AST, GGT, LDH, and ALT), the activity of pro-oxidative enzyme xanthine oxidase, as well as the level of lipid peroxidation in the liver in Group IV compared to Group III (p<0.01). Bilberry extract resulted in a significant increase in the activity of the antioxidant markers-catalase (p<0.05), superoxide dismutase, glutathione S-transferase and glutathione peroxidase (p<0.01), and the concentration of reduced glutathione (p<0.05) in Group IV in relation to Group III. The application of bilberry extract resulted in an increase in the number of apoptotic hepatocytes and the activity of caspase-3 in the liver tissue (p<0.01). The reduction of coagulation necrotic areas was proved (p<0.001) as well as the number of macrovesicular hepatocytes (p<0.01), along with an increased mitotic activity (p<0.01) in Group IV compared to Group III.
Anthocyanins from bilberry extract have strong antioxidant properties and therefore can be considered as powerful hepatoprotectives in natural products.
本研究旨在通过监测氧化应激和细胞凋亡相关参数，进行组织病理学和形态计量学分析，来确定蓝莓提取物花色苷对四氯化碳（CCl4）诱导的大鼠的肝保护作用。
将动物分为四组：I 组（0.9%NaCl-10 天）、II 组（蓝莓提取物，75mg/kg-10 天）、III 组（0.9%NaCl-9 天，第十天 CCl4-2ml/kg）、IV 组（蓝莓提取物，75mg/kg-10 天，第十天 CCl4-2ml/kg）。
与 III 组相比，IV 组血清（AST、GGT、LDH 和 ALT）生化参数活性、黄嘌呤氧化酶等促氧化酶活性以及肝脏脂质过氧化水平均显著降低（p<0.01）。蓝莓提取物可显著提高 IV 组抗氧化标志物（CAT）活性（p<0.05）、超氧化物歧化酶、谷胱甘肽 S-转移酶和谷胱甘肽过氧化物酶（p<0.01）以及还原型谷胱甘肽浓度（p<0.05）。蓝莓提取物的应用导致肝组织中凋亡肝细胞数量和 caspase-3 活性增加（p<0.01）。与 III 组相比，IV 组凝血性坏死面积减少（p<0.001），巨泡性肝细胞数量减少（p<0.01），有丝分裂活性增加（p<0.01）。
蓝莓提取物花色苷具有较强的抗氧化特性，因此可被视为天然产物中强有力的保肝剂。
