Graduate School of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan.
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Nutrients. 2020 Oct 23;12(11):3252. doi: 10.3390/nu12113252.
Non-alcoholic fatty liver disease (NAFLD) is a manifestation of metabolic syndrome closely linked to dyslipidemia and gut microbiome dysbiosis. Bilberry anthocyanins (BA) have been reported to have preventive effects against metabolic syndrome. This study aimed to investigate the protective effects and mechanisms of BA in a Western diet (WD)-induced mouse model. The results revealed that supplementation with BA attenuated the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), low-density lipoprotein cholesterol (LDL-c), fat content in liver, 2-thiobarbituric acid reactive substances (TBARS) and α-smooth muscle actin (α-SMA) caused by WD. Furthermore, gut microbiota characterized by 16S rRNA sequencing revealed that BA reduced remarkably the ratio of Firmicutes/Bacteroidetes (F/B) and modified gut microbiome. In particular, BA increased the relative abundance of g_ and g_. Taken together, our data demonstrated that BA might ameliorate WD-induced NAFLD by attenuating dyslipidemia and gut microbiome dysbiosis.
非酒精性脂肪性肝病 (NAFLD) 是代谢综合征的一种表现形式，与血脂异常和肠道微生物群落失调密切相关。越桔花色苷 (BA) 已被报道具有预防代谢综合征的作用。本研究旨在探讨 BA 在西方饮食 (WD) 诱导的小鼠模型中的保护作用及其机制。结果表明，BA 补充剂可减轻 WD 引起的血清天冬氨酸转氨酶 (AST)、丙氨酸转氨酶 (ALT)、低密度脂蛋白胆固醇 (LDL-c)、肝脏脂肪含量、2-硫代巴比妥酸反应物质 (TBARS) 和 α-平滑肌肌动蛋白 (α-SMA) 的水平。此外，通过 16S rRNA 测序鉴定的肠道微生物组表明，BA 可显著降低厚壁菌门/拟杆菌门 (Firmicutes/Bacteroidetes，F/B) 的比值，并改善肠道微生物组。特别是，BA 增加了 g_ 和 g_ 的相对丰度。综上所述，我们的数据表明，BA 可能通过减轻血脂异常和肠道微生物群落失调来改善 WD 诱导的 NAFLD。
