Khairani Astrid Feinisa, Pamela Yunisa, Oktavia Nandina, Achadiyani Achadiyani, Adipraja M Yusuf, Zhafira Prita Yasri, Shalannandia Widad Aghnia, Atik Nur
Department of Biomedical Sciences, Division of Cell Biology, Faculty of Medicine, Universitas Padjadjaran, West Java, Indonesia.
Department of Biomedical Sciences, Division of Biochemistry and Biology Molecular, Faculty of Medicine, Universitas Padjadjaran, West Java, Indonesia.
Vet World. 2022 Mar;15(3):789-796. doi: 10.14202/vetworld.2022.789-796. Epub 2022 Mar 31.
Food safety is an important aspect to be evaluated in preventing any potentially harmful side effects of food product such as yogurt. The purple sweet potato yogurt product was developed to combine the benefits of probiotic activities in yogurt and the bioactive effects of anthocyanin in purple sweet potato. This study was performed to investigate acute and sub-chronic oral toxicity of purple sweet potato yogurt (PSPY) in mice.
Acute oral toxicity was evaluated by a 14-day observation for any clinical sign of toxicity on fifteen female balb/c mice following a single dosage of PSPY (nil, 2 or 5 g/kg body weight). The sub-chronic oral toxicity study was conducted by feeding PSPY to four groups of mice with the dose of 0, 12, 20, and 40 g/kg body weight for 28 days, and another group of mice receiving 40 g/kg body weight purple sweet potato for 14 days longer to observe any delayed toxicity effect. Body weight and clinical signs of toxicity were observed daily. Liver and kidney macroscopy and relative organ weight, liver histology, liver enzyme, and hematology profile analyses were done at the end of the study.
There were no signs of toxicity observed from the acute toxicity study and no abnormality in body weight, relative organ weight, and gross organ examination. In the sub-chronic toxicity study, there were no clinical signs of toxicity, no significant differences in body weight, relative liver weight, liver enzymes, hematology profile, or abnormality in gross and histological examination of the liver.
This study shows that oral administration of PSPY in mice up to 5 g/kg body weight did not result in acute toxicity, while the dosage up to 40 g/kg body weight did not lead to sub-chronic toxicity.
食品安全是评估食品（如酸奶）潜在有害副作用时的一个重要方面。研发紫甘薯酸奶产品是为了将酸奶中益生菌活性的益处与紫甘薯中花青素的生物活性作用结合起来。本研究旨在调查紫甘薯酸奶（PSPY）对小鼠的急性和亚慢性经口毒性。
通过对15只雌性Balb/c小鼠单次给予PSPY（剂量为零、2或5克/千克体重）后进行14天的观察，评估急性经口毒性，观察任何毒性临床症状。亚慢性经口毒性研究是将PSPY分别以0、12、20和40克/千克体重的剂量喂给四组小鼠，持续28天，另一组小鼠接受40克/千克体重的紫甘薯，持续时间延长14天，以观察任何延迟毒性效应。每天观察体重和毒性临床症状。在研究结束时进行肝脏和肾脏大体检查、相对器官重量、肝脏组织学、肝酶和血液学分析。
急性毒性研究未观察到毒性迹象，体重、相对器官重量和大体器官检查均无异常。在亚慢性毒性研究中，未观察到毒性临床症状，体重、相对肝脏重量、肝酶、血液学分析或肝脏大体和组织学检查均无显著差异。
本研究表明，小鼠经口给予高达5克/千克体重的PSPY不会导致急性毒性，而高达40克/千克体重的剂量不会导致亚慢性毒性。
