Truong Anh Duc, Park Boyeong, Ban Jihye, Hong Yeong Ho
Department of Animal Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea.
Vet Res. 2016 Jun 16;47(1):65. doi: 10.1186/s13567-016-0342-0.
In the present study, we describe the cloning and functional characterization of chicken interleukin 26 (ChIL-26). ChIL-26, a member of the IL-10 cytokine family, induces the production of proinflammatory cytokines by T cells. The ChIL-26 cDNA encodes an 82-amino-acid protein whose amino acid sequence has 22.63, 46.31 and 43.15% homology with human IL-26, pig IL-26 and canary IL-26, respectively. ChIL-26 signals through a heterodimeric receptor complex composed of the IL-20R1 and IL-10R2 chains, which are expressed primarily in the CU91 T cell line as well as CD4(+) and CD8(+) T cells. Recombinant ChIL-26 protein induced Th1 cytokines (IL-16 and IFN-γ), Th2 cytokines (IL-4, IL-6 and IL-10), Th17 cytokines (IL-17A, IL-17D, and IL-17F), and chemokine transcripts (mainly CCL3, CCL4, CCL5, CCL20 and CXCL13) in the CU91 T cell line and in CD4(+) and CD8(+) T cells, however IL-18 was not expressed in the CU91 T cell line. Taken together, the data demonstrates that T cells express the functional ChIL-26 receptor complex and that ChIL-26 modulates T cell proliferation and proinflammatory gene expression. To the best of our knowledge, this is the first report of cloned ChIL-26. We evaluated its functional roles, particularly in the pathogenic costimulation of T cells, which may be significantly associated with the induction of cytokines.
在本研究中，我们描述了鸡白细胞介素26（ChIL-26）的克隆及功能特性。ChIL-26是白细胞介素10细胞因子家族的成员，可诱导T细胞产生促炎细胞因子。ChIL-26 cDNA编码一种82个氨基酸的蛋白质，其氨基酸序列与人白细胞介素26、猪白细胞介素26和金丝雀白细胞介素26的同源性分别为22.63%、46.31%和43.15%。ChIL-26通过由IL-20R1和IL-10R2链组成的异二聚体受体复合物发出信号，这些链主要在CU91 T细胞系以及CD4(+)和CD8(+) T细胞中表达。重组ChIL-26蛋白在CU91 T细胞系以及CD4(+)和CD8(+) T细胞中诱导Th1细胞因子（IL-16和IFN-γ）、Th2细胞因子（IL-4、IL-6和IL-10）、Th17细胞因子（IL-17A、IL-17D和IL-17F）以及趋化因子转录本（主要是CCL3、CCL4、CCL5、CCL20和CXCL13），然而IL-18在CU91 T细胞系中未表达。综上所述，数据表明T细胞表达功能性ChIL-26受体复合物，且ChIL-26调节T细胞增殖和促炎基因表达。据我们所知，这是关于克隆ChIL-26的首次报道。我们评估了其功能作用，特别是在T细胞致病性共刺激中的作用，这可能与细胞因子的诱导显著相关。
