利用 Thy1-GFP 转基因对整个清除的大鼠脑进行光片显微镜成像。

Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.

Institut de Ciencies Fotoniques (ICFO), Barcelona Institute of Science and Technology, 08860, Castelldefels (Barcelona), Spain.

Sci Rep. 2016 Jun 17;6:28209. doi: 10.1038/srep28209.

Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter. We then present data on clearing the rat brain, showing that FluoClearBABB was found superior over passive CLARITY and CUBIC methods. Finally, we demonstrate efficient imaging of the rat brain using light-sheet fluorescence microscopy.

基于光片荧光显微镜和组织透明化的全脑成像技术是一个新兴的快速发展的研究领域。虽然已经成功尝试对小鼠大脑进行了清除和成像，但对于大鼠来说，类似的结果却很难实现。在这里，我们报告创建了一种新型的转基因大鼠，该大鼠在神经元基因启动子的控制下表达荧光报告基因 GFP。然后，我们提供了关于大鼠大脑清除的相关数据，结果表明 FluoClearBABB 比被动 CLARITY 和 CUBIC 方法更优越。最后，我们展示了使用光片荧光显微镜对大鼠大脑进行高效成像的结果。